DDX5 antibody: 9877


Antibody Info
Product_ID9877
Lot_ID1
Host_organismrabbit
SourceCell Signaling Technology
Antibody_DCC_IDENCAB946VDH
Target Info
GeneNameDDX5
Phosphate
Target_nameDDX5-human

Validation Images
Cell linePrimary validationSecondary validation
HepG2 DDX5_HepG2.png<br>Caption: IP-Western Blot analysis of HepG2 whole cell lysate using DDX5 specific antibody. Lane 1 is 1% of ten million whole cell lysate input (lane under '15% input') , lane 2 is 20% of IP enrichment using rabbit normal IgG (lane under 'IgG') and lane 3 is 20% IP enrichment using rabbit monoclonal anti-DROSHA antibody (lanes under 'anti-DDX5'). Asterisk indicates heavy chain of antibody.

Caption: IP-Western Blot analysis of HepG2 whole cell lysate using DDX5 specific antibody. Lane 1 is 1% of ten million whole cell lysate input (lane under '15% input') , lane 2 is 20% of IP enrichment using rabbit normal IgG (lane under 'IgG') and lane 3 is 20% IP enrichment using rabbit monoclonal anti-DROSHA antibody (lanes under 'anti-DDX5'). Asterisk indicates heavy chain of antibody.

Method: immunoprecipitation

Status: Released

Lab: Fu Lab

DDX5-HEPG2-FU%27S.png<br>Caption: Western blot following shRNA against DDX5 in HepG2 whole cell lysate using DDX5 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against DDX5. DDX5 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.

Caption: Western blot following shRNA against DDX5 in HepG2 whole cell lysate using DDX5 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against DDX5. DDX5 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.

Releated Sample: BGHLV30-25

Status: Released

Lab: Graveley Lab