EIF2AK1 antibody: 711581


Antibody Info
Product_ID711581
Lot_ID2035751
Host_organismrabbit
SourceThermo Fisher
Antibody_DCC_IDENCAB357SKW
Target Info
GeneNameEIF2AK1
Phosphate
Target_nameEIF2AK1-human

Validation Images
Cell linePrimary validationSecondary validation
K562 ThermoFisher_711581_2035751_EIF2AK1.png<br>Caption: IP-WB analysis of K562 whole cell lysate using the EIF2AK1 specific antibody, 711581. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the EIF2AK1-specific antibody, 711581. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.

Caption: IP-WB analysis of K562 whole cell lysate using the EIF2AK1 specific antibody, 711581. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the EIF2AK1-specific antibody, 711581. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.

Method: immunoprecipitation

Status: Released

Lab: Yeo Lab