AGO1 antibody: 5053


Antibody Info
Product_ID5053
Lot_ID1
Host_organismrabbit
SourceCell Signaling Technology
Antibody_DCC_IDENCAB189GDE
Target Info
GeneNameAGO1
Phosphate
Target_nameAGO1-human

Validation Images
Cell linePrimary validationSecondary validation
HepG2 AGO1_HepG2.png<br>Caption: IP-Western Blot analysis of HepG2 whole cell lysate using AGO1/EIF2C1 specific antibody. Lane 1 is 3% of ten million whole cell lysate input (lane under '15% input') , lane 2 is 20% of IP enrichment using rabbit normal IgG (lane under 'IgG') and lane 3 is 20% IP enrichment using rabbit monoclonal anti-AGO1/EIF2C11 antibody (lanes under 'anti-AGO1'). Asterisk indicates heavy chain of antibody.

Caption: IP-Western Blot analysis of HepG2 whole cell lysate using AGO1/EIF2C1 specific antibody. Lane 1 is 3% of ten million whole cell lysate input (lane under '15% input') , lane 2 is 20% of IP enrichment using rabbit normal IgG (lane under 'IgG') and lane 3 is 20% IP enrichment using rabbit monoclonal anti-AGO1/EIF2C11 antibody (lanes under 'anti-AGO1'). Asterisk indicates heavy chain of antibody.

Method: immunoprecipitation

Status: Released

Lab: Fu Lab

EIF2C1-HEPG2-FU%27S.png<br>Caption: Western blot following shRNA against EIF2C1 in HepG2 whole cell lysate using EIF2C1 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against EIF2C1. EIF2C1 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.

Caption: Western blot following shRNA against EIF2C1 in HepG2 whole cell lysate using EIF2C1 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against EIF2C1. EIF2C1 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.

Releated Sample: BGHLV14-13

Status: Released

Lab: Graveley Lab

K562 AGO1_K562.png<br>Caption: IP-Western Blot analysis of K562 whole cell lysate using AGO1/EIF2C1 specific antibody. Lane 1 is 3% of ten million whole cell lysate input (lane under '15% input') , lane 2 is 20% of IP enrichment using rabbit normal IgG (lane under 'IgG') and lane 3 is 20% IP enrichment using rabbit monoclonal anti-AGO1/EIF2C11 antibody (lanes under 'anti-AGO1'). Asterisk indicates heavy chain of antibody.

Caption: IP-Western Blot analysis of K562 whole cell lysate using AGO1/EIF2C1 specific antibody. Lane 1 is 3% of ten million whole cell lysate input (lane under '15% input') , lane 2 is 20% of IP enrichment using rabbit normal IgG (lane under 'IgG') and lane 3 is 20% IP enrichment using rabbit monoclonal anti-AGO1/EIF2C11 antibody (lanes under 'anti-AGO1'). Asterisk indicates heavy chain of antibody.

Method: immunoprecipitation

Status: Released

Lab: Fu Lab