SRSF3 antibody: 33-4200


Antibody Info
Product_ID33-4200
Lot_IDTE268372
Host_organismmouse
SourceThermo Fisher
Antibody_DCC_IDENCAB889VRL
Target Info
GeneNameSRSF3
Phosphate
Target_nameSRSF3-human

Validation Images
Cell linePrimary validationSecondary validation
HepG2 SRSF3-HEPG2-33-4200.png<br>Caption: Western blot following shRNA against SRSF3 in HepG2 whole cell lysate using SRSF3 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different shRNA against SRSF3. SRSF3 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.

Caption: Western blot following shRNA against SRSF3 in HepG2 whole cell lysate using SRSF3 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different shRNA against SRSF3. SRSF3 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.

Releated Sample: BGHLV35-49

Status: Released

Lab: Graveley Lab

K562 ThermoFisher_33-4200_TE268372_SRSF3.png<br>Caption: IP-WB analysis of K562 whole cell lysate using the SRSF3 specific antibody, 33-4200. Lanes 1 and 3 are 2.5% of five million whole cell lysate input. Lanes 2 and 4 are 50% of IP enrichment from five million whole cell lysate using normal IgG antibody and the SRSF3-specific antibody, 33-4200. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.

Caption: IP-WB analysis of K562 whole cell lysate using the SRSF3 specific antibody, 33-4200. Lanes 1 and 3 are 2.5% of five million whole cell lysate input. Lanes 2 and 4 are 50% of IP enrichment from five million whole cell lysate using normal IgG antibody and the SRSF3-specific antibody, 33-4200. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.

Method: immunoprecipitation

Status: Released

Lab: Yeo Lab