SUPT6H antibody: 15616S


Antibody Info
Product_ID15616S
Lot_ID1
Host_organismrabbit
SourceCell Signaling Technology
Antibody_DCC_ID
Target Info
GeneNameSUPT6H
Phosphate
Target_nameSUPT6H-human

Validation Images
Cell linePrimary validationSecondary validation
HepG2 SUPT6H-HEPG2-15616S.png<br>Caption: Western blot following shRNA against SUPT6H in HepG2 whole cell lysate using SUPT6H specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different shRNA against SUPT6H. SUPT6H protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.

Caption: Western blot following shRNA against SUPT6H in HepG2 whole cell lysate using SUPT6H specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different shRNA against SUPT6H. SUPT6H protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.

Releated Sample: BGHLV30-53

Status: Submitted

Lab: Graveley Lab

K562 CST_15616S_1_SUPT6H.png<br>Caption: IP-WB analysis of 15616S whole cell lysate using the SUPT6H specific antibody, 15616S. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the SUPT6H-specific antibody, 15616S. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.

Caption: IP-WB analysis of 15616S whole cell lysate using the SUPT6H specific antibody, 15616S. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the SUPT6H-specific antibody, 15616S. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.

Method: immunoprecipitation

Status: Submitted

Lab: Yeo Lab