Antibodies of SRSF1


2 antibodies are included.     

 Released    NotSatisfied    Submitted    NotReviewed    OnHold    Submitting    Experimented   
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
SRSF1Product_ID: A302-052A
Lot_ID: 1
Source: Bethyl Labs
Target Name: SRSF1-human		 HepG2_Bethyl_A302-052A_1_SRSF1.png<br>Caption: IP-Western Blot analysis of HepG2 whole cell lysate using SRSF1 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 10% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-ASF (SRSF1) antibody (lanes under 'SRSF1').
 SRSF1_Secondary_Western.png<br>Caption: Western blot following shRNA against SRSF1 in K562 and HepG2 whole cell lysate using SRSF1 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against SRSF1. Lanes 5-8 follow the same pattern, but in HepG2. SRSF1 protein appears as the green band, Tubulin serves as a control and appears in red.
 K562_Bethyl_A302-052A_1_SRSF1.png<br>Caption: IP-Western Blot analysis of K562 whole cell lysate using SRSF1 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 10% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-ASF (SRSF1) antibody (lanes under 'SRSF1').
 SRSF1_Secondary_Western.png<br>Caption: Western blot following shRNA against SRSF1 in K562 and HepG2 whole cell lysate using SRSF1 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against SRSF1. Lanes 5-8 follow the same pattern, but in HepG2. SRSF1 protein appears as the green band, Tubulin serves as a control and appears in red.
SRSF1Product_ID: 32-4500
Lot_ID: TG273235
Source: Thermo Fisher
Target Name: SRSF1-human		 SRSF1-HepG2.png<br>Caption: Western blot following shRNA against SRSF1 in HepG2 whole cell lysate using SRSF1 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different shRNA against SRSF1. SRSF1 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
 ThermoFisher_32-4500_TG273235_SRSF1.png<br>Caption: IP-WB analysis of K562 whole cell lysate using the SRSF1 specific antibody, 32-4500. Lanes 1 and 3 are 2.5% of five million whole cell lysate input. Lanes 2 and 4 are 50% of IP enrichment from five million whole cell lysate using normal IgG antibody and the SRSF1-specific antibody, 32-4500. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.