Antibodies of SMAD5


1 antibodies are included.     

 Released    NotSatisfied    Submitted    NotReviewed    OnHold    Submitting    Experimented   
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
SMAD5Product_ID: 39-5700
Lot_ID: SA247535
Source: Thermo Fisher
Target Name: SMAD5-human
SMAD5-HEPG2-CRISPR-39-5700.png<br>Caption: Western blot following CRISPR against SMAD5 in HepG2 whole cell lysate using SMAD5 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against SMAD5. SMAD5 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.
ThermoFisher_39-5700_SA247535_SMAD5.png<br>Caption: IP-WB analysis of K562 whole cell lysate using the SMAD5 specific antibody, 39-5700. Lane 1 is 2.5% of five million whole cell lysate input. Lanes 2 and 3 are 50% of IP enrichment from five million whole cell lysate using normal IgG antibody and the SMAD5-specific antibody, 39-5700. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
SMAD5-K562-CRISPR-39-5700.png<br>Caption: Western blot following CRISPR against SMAD5 in K562 whole cell lysate using SMAD5 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against SMAD5. SMAD5 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.