Antibodies of SMAD4


1 antibodies are included.     

 Released    NotSatisfied    Submitted    NotReviewed    OnHold    Submitting    Experimented   
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
SMAD4Product_ID: 701682
Lot_ID: 2023880
Source: Thermo Fisher
Target Name: SMAD4-human		 SMAD4-HEPG2-CRISPR-701682.png<br>Caption: Western blot following CRISPR against SMAD4 in HepG2 whole cell lysate using SMAD4 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against SMAD4. SMAD4 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
 ThermoFisher_701682_2023880_SMAD4.png<br>Caption: IP-WB analysis of K562 whole cell lysate using the SMAD4 specific antibody, 701682. Lane 1 is 2.5% of five million whole cell lysate input. Lanes 2 and 3 are 50% of IP enrichment from five million whole cell lysate using normal IgG antibody and the SMAD4-specific antibody, 701682. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
 SMAD4-K562-CRISPR-701682.png<br>Caption: Western blot following CRISPR against SMAD4 in K562 whole cell lysate using SMAD4 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against SMAD4. SMAD4 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.