Antibodies of SF3B2


2 antibodies are included.     

 Released    NotSatisfied    Submitted    NotReviewed    OnHold    Submitting    Experimented   
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
SF3B2Product_ID: A301-605A
Lot_ID: 1
Source: Bethyl Labs
Target Name: SF3B2-human
SF3B2-HEPG2-CRISPR-A301-605A.png<br>Caption: Western blot following CRISPR against SF3B2 in HepG2 whole cell lysate using SF3B2 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against SF3B2. SF3B2 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
Bethyl_A301-605A_1_SF3B2.png<br>Caption: IP-WB analysis of K562 whole cell lysate using the SF3B2 specific antibody, A301-605A. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the SF3B2-specific antibody, A301-605A. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
SF3B2-K562-CRISPR-A301-605A.png<br>Caption: Western blot following CRISPR against SF3B2 in K562 whole cell lysate using SF3B2 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against SF3B2. SF3B2 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
SF3B2Product_ID: A301-606A
Lot_ID: 1
Source: Bethyl Labs
Target Name: SF3B2-human
Bethyl_A301-606A_1_SF3B2.png<br>Caption: IP-WB analysis of K562 whole cell lysate using the SF3B2 specific antibody, A301-606A. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the SF3B2-specific antibody, A301-606A. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
SF3B2-K562-CRISPR-A301-606A.png<br>Caption: Western blot following CRISPR against SF3B2 in K562 whole cell lysate using SF3B2 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against SF3B2. SF3B2 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.