Antibodies of SCAF8


2 antibodies are included.     

 Released    NotSatisfied    Submitted    NotReviewed    OnHold    Submitting    Experimented   
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
SCAF8Product_ID: A301-036A
Lot_ID: 1
Source: Bethyl Labs
Target Name: SCAF8-human		 SCAF8-HEPG2-CRISPR-A301-036A.png<br>Caption: Western blot following CRISPR against SCAF8 in HepG2 whole cell lysate using SCAF8 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against SCAF8. SCAF8 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
 Bethyl_A301-036A_1_SCAF8.png<br>Caption: IP-WB analysis of K562 whole cell lysate using the SCAF8 specific antibody, A301-036A. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the SCAF8-specific antibody, A301-036A. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
 SCAF8-K562-CRISPR-A301-036A.png<br>Caption: Western blot following CRISPR against SCAF8 in K562 whole cell lysate using SCAF8 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against SCAF8. SCAF8 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
SCAF8Product_ID: A301-037A
Lot_ID: 1
Source: Bethyl Labs
Target Name: SCAF8-human		 Bethyl_A301-037A_1_SCAF8.png<br>Caption: IP-WB analysis of K562 whole cell lysate using the SCAF8 specific antibody, A301-036A. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the SCAF8-specific antibody, A301-036A. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
 SCAF8-K562-CRISPR-A301-037A.png<br>Caption: Western blot following CRISPR against SCAF8 in K562 whole cell lysate using SCAF8 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against SCAF8. SCAF8 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.