Antibodies of SART3


3 antibodies are included.     

 Released    NotSatisfied    Submitted    NotReviewed    OnHold    Submitting    Experimented   
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
SART3Product_ID: GTX107684
Lot_ID: 39757
Source: GeneTex
Target Name: SART3-human		 HepG2_Genetex_GT107684_39757_SART3.png<br>Caption: IP-Western Blot analysis of HepG2 whole cell lysate using SART3 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-SART3 antibody (lanes under 'SART3').
 SART3_Secondary_Western.png<br>Caption: Western blot following shRNA against SART3 in K562 and HepG2 whole cell lysate using SART3 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against SART3. Lanes 5-8 follow the same pattern, but in HepG2. SART3 protein appears as the green band, Tubulin serves as a control and appears in red.
 SART3_Secondary_Western.png<br>Caption: Western blot following shRNA against SART3 in K562 and HepG2 whole cell lysate using SART3 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against SART3. Lanes 5-8 follow the same pattern, but in HepG2. SART3 protein appears as the green band, Tubulin serves as a control and appears in red.
SART3Product_ID: A301-521A
Lot_ID: 1
Source: Bethyl Labs
Target Name: SART3-human		 SART3-HEPG2-A301-521A.png<br>Caption: Western blot following shRNA against SART3 in HepG2 whole cell lysate using SART3 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different shRNA against SART3. SART3 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
 Bethyl_A301-521A_1_SART3.png<br>Caption: IP-WB analysis of K562 whole cell lysate using the SART3 specific antibody, A301-521A. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the SART3-specific antibody, A301-521A. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
 SART3-K562-A301-521A.png<br>Caption: Western blot following shRNA against SART3 in K562 whole cell lysate using SART3 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNA against SART3. SART3 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
SART3Product_ID: A301-522A
Lot_ID: 1
Source: Bethyl Labs
Target Name: SART3-human		 SART3-HEPG2-A301-522A.png<br>Caption: Western blot following shRNA against SART3 in HepG2 whole cell lysate using SART3 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different shRNA against SART3. SART3 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
 Bethyl_A301-522A_1_SART3.png<br>Caption: IP-WB analysis of K562 whole cell lysate using the SART3 specific antibody, A301-522A. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the SART3-specific antibody, A301-522A. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.