Antibodies of SARNP


2 antibodies are included.     

 Released    NotSatisfied    Submitted    NotReviewed    OnHold    Submitting    Experimented   
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
SARNPProduct_ID: A303-209A
Lot_ID: 1
Source: Bethyl Labs
Target Name: SARNP-human		 HepG2_Bethyl_A303-209A_1_CIP29.png<br>Caption: IP-Western Blot analysis of HepG2 whole cell lysate using CIP29 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-CIP29 antibody (lanes under 'CIP29').
 RPS2_Secondary_Western.png<br>Caption: Western blot following shRNA against SARNP in HepG2 whole cell lysate using SARNP specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different shRNA against SARNP. SARNP protein appears as the green arrow, Tubulin serves as a control and appears in red arrow.
SARNP-HEPG2-CRISPR-A303-209A.png<br>Caption: Western blot following CRISPR against SARNP in HepG2 whole cell lysate using SARNP specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against SARNP. SARNP protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
 K562_Bethyl_A303-209A_1_CIP29.png<br>Caption: IP-Western Blot analysis of K562 whole cell lysate using CIP29 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-CIP29 antibody (lanes under 'CIP29').
Bethyl_A303-209A_1_SARNP.png<br>Caption: IP-WB analysis of K562 whole cell lysate using the SARNP specific antibody, A303-209A. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the SARNP-specific antibody, A303-209A. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
 SARNP-K562-CRISPR-A303-209A.png<br>Caption: Western blot following CRISPR against SARNP in K562 whole cell lysate using SARNP specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against SARNP. SARNP protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
SARNPProduct_ID: A303-210A
Lot_ID: 1
Source: Bethyl Labs
Target Name: SARNP-human		 Bethyl_A303-210A_1_SARNP.png<br>Caption: IP-WB analysis of K562 whole cell lysate using the SARNP specific antibody, A303-210A. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the SARNP-specific antibody, A303-210A. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
 SARNP-K562-CRISPR-A303-210A.png<br>Caption: Western blot following CRISPR against SARNP in K562 whole cell lysate using SARNP specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against SARNP. SARNP protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.