Antibodies of RTF1


3 antibodies are included.     

 Released    NotSatisfied    Submitted    NotReviewed    OnHold    Submitting    Experimented   
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
RTF1Product_ID: A300-178A
Lot_ID: 1
Source: Bethyl Labs
Target Name: RTF1-human		 RTF1-hepg2.png<br>Caption: Western blot following shRNA against RTF1 in HepG2 whole cell lysate using RTF1 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against RTF1. RTF1 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.
 K562_Bethyl_A300-178A_1_RTF1.png<br>Caption: IP-Western Blot analysis of K562 whole cell lysate using RTF1 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-RTF1 antibody (lanes under 'RTF1').
 RTF1-K562.png<br>Caption: Western blot following shRNA against RTF1 in K562 whole cell lysate using RTF1 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against RTF1. RTF1 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.
RTF1Product_ID: A300-179A
Lot_ID: 1
Source: Bethyl Labs
Target Name: RTF1-human		 RTF1-HEPG2-A300-179A.png<br>Caption: Western blot following shRNA against RTF1 in HepG2 whole cell lysate using RTF1 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different shRNA against RTF1. RTF1 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
 Bethyl_A300-179A_1_RTF1.png<br>Caption: IP-WB analysis of K562 whole cell lysate using the RTF1 specific antibody, A300-179A. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the RTF1-specific antibody, A300-179A. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
RTF1Product_ID: A301-329A
Lot_ID: 1
Source: Bethyl Labs
Target Name: RTF1-human		 RTF1-HEPG2-A301-329A.png<br>Caption: Western blot following shRNA against RTF1 in HepG2 whole cell lysate using RTF1 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different shRNA against RTF1. RTF1 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
 Bethyl_A301-329A_1_RTF1.png<br>Caption: IP-WB analysis of K562 whole cell lysate using the RTF1 specific antibody, A301-329A. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the RTF1-specific antibody, A301-329A. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.