Antibodies of RPS6


4 antibodies are included.     

 Released    NotSatisfied    Submitted    NotReviewed    OnHold    Submitting    Experimented   
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
RPS6Product_ID: A300-556A
Lot_ID: 2
Source: Bethyl Labs
Target Name: RPS6-human
Bethyl_A300-556A_2_RPS6.png<br>Caption: IP-WB analysis of K562 whole cell lysate using the RPS6 specific antibody, A300-556A. Lanes 1 and 3 are 2.5% of five million whole cell lysate input. Lanes 2 and 4 are 50% of IP enrichment from five million whole cell lysate using normal IgG antibody and the RPS6-specific antibody, A300-556A. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
RPS6-K562-CRISPR.png<br>Caption: Western blot following CRISPR against RPS6 in K562 whole cell lysate using RPS6 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against RPS6. RPS6 protein appears as the green arrow, Tubulin serves as a control and appears in red arrow.
RPS6-K562-CRISPR-A300-556A.png<br>Caption: Western blot following CRISPR against RPS6 in K562 whole cell lysate using RPS6 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against RPS6. RPS6 protein appears as the green arrow, Tubulin serves as a control and appears in red arrow.
RPS6Product_ID: A300-557A
Lot_ID: 2
Source: Bethyl Labs
Target Name: RPS6-human
Bethyl_A300-557A_2_RPS6.png<br>Caption: IP-WB analysis of K562 whole cell lysate using the RPS6 specific antibody, A300-557A. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the RPS6-specific antibody, A300-557A. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
RPS6-K562-CRISPR-A300-557A.png<br>Caption: Western blot following CRISPR against RPS6 in K562 whole cell lysate using RPS6 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against RPS6. RPS6 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
RPS6Product_ID: 701845
Lot_ID: RF236380
Source: Thermo Fisher
Target Name: RPS6phosphoS240phosphoS244-human
Phosphate: pSer240pSer244
ThermoFisher_701845_RF236380_RPS6pSer240-244.png<br>Caption: IP-WB analysis of K562 whole cell lysate using the RPS6 pSer240, Ser244 specific antibody, 701845. Lanes 1 and 3 are 2.5% of five million whole cell lysate input. Lanes 2 and 4 are 50% of IP enrichment from five million whole cell lysate using normal IgG antibody and the RPS6 pSer240, Ser244-specific antibody, 701845. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
RPS6-pSer240pSer244-K562-CRISPR-701845.png<br>Caption: Western blot following CRISPR against RPS6-pSer240pSer244 in K562 whole cell lysate using RPS6-pSer240pSer244 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against RPS6-pSer240pSer244. RPS6-pSer240pSer244 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
RPS6Product_ID: 701374
Lot_ID: TH2627537A
Source: Thermo Fisher
Target Name: RPS6-human
RPS6-K562-CRISPR-701374.png<br>Caption: Western blot following CRISPR against RPS6 in K562 whole cell lysate using RPS6 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against RPS6. RPS6 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.