Antibodies of RBM5


2 antibodies are included.     

 Released    NotSatisfied    Submitted    NotReviewed    OnHold    Submitting    Experimented   
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
RBM5Product_ID: A302-228A
Lot_ID: 1
Source: Bethyl Labs
Target Name: RBM5-human
HepG2_Bethyl_A302-228A_1_RBM5.png<br>Caption: IP-Western Blot analysis of HepG2 whole cell lysate using RBM5 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-RBM5 antibody (lanes under 'RBM5').
RBM5-HEPG2-CRISPR.png<br>Caption: Western blot following CRISPR against RBM5 in HepG2 whole cell lysate using RBM5 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against RBM5. RBM5 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.
RBM5Product_ID: 86425S
Lot_ID: 1
Source: Cell Signaling Technology
Target Name: RBM5-human
CST_86425S_1_RBM5.png<br>Caption: IP-WB analysis of 86425S whole cell lysate using the RBM5 specific antibody, 86425S. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the RBM5-specific antibody, 86425S. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
RBM5-K562-CRISPR-86425S.png<br>Caption: Western blot following CRISPR against RBM5 in K562 whole cell lysate using RBM5 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against RBM5. RBM5 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.