Antibodies of RBM14


3 antibodies are included.     

 Released    NotSatisfied    Submitted    NotReviewed    OnHold    Submitting    Experimented   
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
RBM14Product_ID: A300-331A
Lot_ID: 1
Source: Bethyl Labs
Target Name: RBM14-human
Bethyl_A300-331A_1_RBM14.png<br>Caption: IP-WB analysis of K562 whole cell lysate using the RBM14 specific antibody, A300-331A. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the RBM14-specific antibody, A300-331A. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
RBM14-K562-CRISPR-A300-331A.png<br>Caption: Western blot following CRISPR against RBM14 in K562 whole cell lysate using RBM14 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against RBM14. RBM14 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
RBM14Product_ID: A300-845A
Lot_ID: 2
Source: Bethyl Labs
Target Name: RBM14-human
Bethyl_A300-845A_2_RBM14.png<br>Caption: IP-WB analysis of K562 whole cell lysate using the RBM14 specific antibody, A300-845A. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the RBM14-specific antibody, A300-845A. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
RBM14-K562-CRISPR-A300-845A.png<br>Caption: Western blot following CRISPR against RBM14 in K562 whole cell lysate using RBM14 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against RBM14. RBM14 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
RBM14Product_ID: RN069PW
Lot_ID: 1
Source: MBLI
Target Name: RBM14-human
RBM14_HepG2.png<br>Caption: IP-Western Blot analysis of HepG2 whole cell lysate using RBM14 specific antibody. Lane 1 is 4% of ten million whole cell lysate input (lane under '20% input') , lane 2 is 20% of IP enrichment using rabbit normal IgG (lane under 'IgG') and lane 3 is 20% IP enrichment using rabbit monoclonal anti-RBM14 antibody (lanes under 'anti-RBM14'). Asterisk indicates heavy chain of antibody.
RBM14-CRISPR-HEPG2.png<br>Caption: Western blot following CRISPR against RBM14 in HepG2 whole cell lysate using RBM14 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against RBM14. RBM14 protein appears as the green arrow, Tubulin serves as a control and appears in red arrow.
RBM14_K562.png<br>Caption: IP-Western Blot analysis of K562 whole cell lysate using RBM14 specific antibody. Lane 1 is 1% of ten million whole cell lysate input (lane under '5% input') , lane 2 is 20% of IP enrichment using rabbit normal IgG (lane under 'IgG') and lane 3 is 20% IP enrichment using rabbit monoclonal anti-RBM14 antibody (lanes under 'anti-RBM14'). Asterisk indicates heavy chain of antibody.