POLR2A | Product_ID: MA1-46093
Lot_ID: SC249656
Source: Thermo Fisher
Target Name: POLR2A-human | | | ![ThermoFisher_MA1-46093_SC249656_POLR2A.png<br>Caption: IP-WB analysis of K562 whole cell lysate using the POLR2A specific antibody, MA1-46093. Lanes 1 and 3 are 2.5% of five million whole cell lysate input. Lanes 2 and 4 are 50% of IP enrichment from five million whole cell lysate using normal IgG antibody and the POLR2A-specific antibody, MA1-46093. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.](https://graveleylab.cam.uchc.edu/ENCODE/ENCODE_DATA/WesternBlot_primary/ThermoFisher_MA1-46093_SC249656_POLR2A.png)
| ![POLR2A-K562-CRISPR-MA1-46093.png<br>Caption: Western blot following CRISPR against POLR2A in K562 whole cell lysate using POLR2A specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against POLR2A. POLR2A protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.](https://graveleylab.cam.uchc.edu/ENCODE/ENCODE_DATA/WesternBlot/POLR2A-K562-CRISPR-MA1-46093.png)
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POLR2A | Product_ID: 61083
Lot_ID: 22217002
Source: Active Motif
Target Name: POLR2AphosphoS2-human Phosphate: pSer2 | | | | ![POLR2A-pSer2-K562-CRISPR-61083.png<br>Caption: Western blot following CRISPR against POLR2A-pSer2 in K562 whole cell lysate using POLR2A-pSer2 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against POLR2A-pSer2. POLR2A-pSer2 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.](https://graveleylab.cam.uchc.edu/ENCODE/ENCODE_DATA/WesternBlot/POLR2A-pSer2-K562-CRISPR-61083.png)
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POLR2A | Product_ID: 61085
Lot_ID: 10618002
Source: Active Motif
Target Name: POLR2AphosphoS5-human Phosphate: pSer5 | | | | ![POLR2A-pSer5-K562-CRISPR-61085.png<br>Caption: Western blot following CRISPR against POLR2A-pSer5 in K562 whole cell lysate using POLR2A-pSer5 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against POLR2A-pSer5. POLR2A-pSer5 protein appears as the green arrow, Tubulin serves as a control and appears in red arrow.](https://graveleylab.cam.uchc.edu/ENCODE/ENCODE_DATA/WesternBlot/POLR2A-pSer5-K562-CRISPR-61085.png)
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POLR2A | Product_ID: 61087
Lot_ID: 15117003
Source: Active Motif
Target Name: POLR2AphosphoS7-human Phosphate: pSer7 | | | | ![POLR2A-pSer7-K562-CRISPR-61087.png<br>Caption: Western blot following CRISPR against POLR2A-pSer7 in K562 whole cell lysate using POLR2A-pSer7 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against POLR2A-pSer7. POLR2A-pSer7 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.](https://graveleylab.cam.uchc.edu/ENCODE/ENCODE_DATA/WesternBlot/POLR2A-pSer7-K562-CRISPR-61087.png)
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POLR2A | Product_ID: 61361
Lot_ID: 22217002
Source: Active Motif
Target Name: POLR2AphosphoT4-human Phosphate: pThr4 | | | | ![POLR2A-pThr4-K562-CRISPR-61361.png<br>Caption: Western blot following CRISPR against POLR2A-pThr4 in K562 whole cell lysate using POLR2A-pThr4 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against POLR2A-pThr4. POLR2A-pThr4 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.](https://graveleylab.cam.uchc.edu/ENCODE/ENCODE_DATA/WesternBlot/POLR2A-pThr4-K562-CRISPR-61361.png)
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POLR2A | Product_ID: 61383
Lot_ID: 31516002
Source: Active Motif
Target Name: POLR2AphosphoY1-human Phosphate: pTyr1 | | | | ![POLR2A-pTyr1-K562-CRISPR-61383.png<br>Caption: Western blot following CRISPR against POLR2A-pTyr1 in K562 whole cell lysate using POLR2A-pTyr1 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against POLR2A-pTyr1. POLR2A-pTyr1 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.](https://graveleylab.cam.uchc.edu/ENCODE/ENCODE_DATA/WesternBlot/POLR2A-pTyr1-K562-CRISPR-61383.png)
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