Antibodies of PDCD4


2 antibodies are included.     

 Released    NotSatisfied    Submitted    NotReviewed    OnHold    Submitting    Experimented   
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
PDCD4Product_ID: PA5-20309
Lot_ID: TK2671263A
Source: Thermo Fisher
Target Name: PDCD4-human		 PDCD4-HEPG2-CRISPR-PA5-20309.png<br>Caption: Western blot following CRISPR against PDCD4 in HepG2 whole cell lysate using PDCD4 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against PDCD4. PDCD4 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
 ThermoFisher_PA5-20309_TK2671263A_PDCD4.png<br>Caption: IP-WB analysis of K562 whole cell lysate using the PDCD4 specific antibody, PA5-20309. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the PDCD4-specific antibody, PA5-20309. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
 PDCD4-K562-CRISPR-PA5-20309.png<br>Caption: Western blot following CRISPR against PDCD4 in K562 whole cell lysate using PDCD4 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against PDCD4. PDCD4 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
PDCD4Product_ID: 9535S
Lot_ID: 5
Source: Cell Signaling Technology
Target Name: PDCD4-human		 CST_9535S_5_PDCD4.png<br>Caption: IP-WB analysis of 9535S whole cell lysate using the PDCD4 specific antibody, 9535S. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the PDCD4-specific antibody, 9535S. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
 PDCD4-K562-CRISPR-9535S.png<br>Caption: Western blot following CRISPR against PDCD4 in K562 whole cell lysate using PDCD4 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against PDCD4. PDCD4 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.