Antibodies of PARP1


3 antibodies are included.     

 Released    NotSatisfied    Submitted    NotReviewed    OnHold    Submitting    Experimented   
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
PARP1Product_ID: 436400
Lot_ID: TE268344
Source: Thermo Fisher
Target Name: PARP1-human		 ThermoFisher_436400_TE268344_PARP.png<br>Caption: IP-WB analysis of K562 whole cell lysate using the PARP specific antibody, 436400. Lanes 1 and 3 are 2.5% of five million whole cell lysate input. Lanes 2 and 4 are 50% of IP enrichment from five million whole cell lysate using normal IgG antibody and the PARP-specific antibody, 436400. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
PARP1Product_ID: 5625S
Lot_ID: 1
Source: Cell Signaling
Target Name: PARP1-human		 CST_5625S_13_PARP1.png<br>Caption: IP-WB analysis of 5625S whole cell lysate using the PARP1 specific antibody, 5625S. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the PARP1-specific antibody, 5625S. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
 PARP1-K562-CRISPR-5625S.png<br>Caption: Western blot following CRISPR against PARP1 in K562 whole cell lysate using PARP1 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against PARP1. PARP1 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
PARP1Product_ID: 9532S
Lot_ID: 10
Source: Cell Signaling Technology
Target Name: PARP1-human		 CST_9532S_10_PARP1.png<br>Caption: IP-WB analysis of 9532S whole cell lysate using the PARP1 specific antibody, 9532S. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the PARP1-specific antibody, 9532S. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
 PARP1-K562-CRISPR-9532S.png<br>Caption: Western blot following CRISPR against PARP1 in K562 whole cell lysate using PARP1 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against PARP1. PARP1 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.