Antibodies of PABPN1


2 antibodies are included.     

 Released    NotSatisfied    Submitted    NotReviewed    OnHold    Submitting    Experimented   
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
PABPN1Product_ID: RN023PW
Lot_ID: 1
Source: MBLI
Target Name: PABPN1-human		 HepG2_MBL_RN023PW_001_PABPN1.png<br>Caption: IP-Western Blot analysis of HepG2 whole cell lysate using PABPN1 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-PABPN1 antibody (lanes under 'PABPN1').
 PABPN1_Secondary_Western.png<br>Caption: Western blot following shRNA against PABPN1 in K562 whole cell lysate using PABPN1 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against PABPN1. PABPN1 protein appears as the green band, Tubulin serves as a control and appears in red.
PABPN1Product_ID: 14154S
Lot_ID: 1
Source: Cell Signaling Technology
Target Name: PABPN1-human		 PABPN1-HEPG2-CRISPR-14154S.png<br>Caption: Western blot following CRISPR against PABPN1 in HepG2 whole cell lysate using PABPN1 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against PABPN1. PABPN1 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
 CST_14154S_1_PABPN1.png<br>Caption: IP-WB analysis of 14154S whole cell lysate using the PABPN1 specific antibody, 14154S. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the PABPN1-specific antibody, 14154S. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
 PABPN1-K562-CRISPR-14154S.png<br>Caption: Western blot following CRISPR against PABPN1 in K562 whole cell lysate using PABPN1 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against PABPN1. PABPN1 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.