Antibodies of NUP35


1 antibodies are included.     

 Released    NotSatisfied    Submitted    NotReviewed    OnHold    Submitting    Experimented   
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
NUP35Product_ID: A301-781A
Lot_ID: 1
Source: Bethyl Labs
Target Name: NUP35-human		 NUP35-HEPG2_Secondary_Western.png<br>Caption: Western blot following shRNA against NUP35 in HepG2 whole cell lysate using NUP35 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against NUP35.NUP35 protein appears as the green band, Tubulin serves as a control and appears in red.
 Bethyl_A301-781A_2_NUP35.png<br>Caption: IP-WB analysis of K562 whole cell lysate using the NUP35 specific antibody, A301-781A. Lane 1 is 2.5% of five million whole cell lysate input. Lanes 2 and 3 are 50% of IP enrichment from five million whole cell lysate using normal IgG antibody and the NUP35-specific antibody, A301-781A. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
 NUP35-k562.png<br>Caption: Western blot following shRNA against NUP35 in k562 whole cell lysate using NUP35 specific antibody. Lane 1 is a ladder, lane 2 is k562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against NUP35. NUP35 protein appears as the green band, Tubulin serves as a control and appears in red.
NUP35-K562-A301-781A.png<br>Caption: Western blot following shRNA against NUP35 in K562 whole cell lysate using NUP35 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNA against NUP35. NUP35 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.