Antibodies of NPM1


3 antibodies are included.     

 Released    NotSatisfied    Submitted    NotReviewed    OnHold    Submitting    Experimented   
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
NPM1Product_ID: A302-402A
Lot_ID: 1
Source: Bethyl Labs
Target Name: NPM1-human		 NPM1_Secondary_Western.png<br>Caption: Western blot following shRNA against NPM1 in K562 and HepG2 whole cell lysate using NPM1 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against NPM1. Lanes 5-8 follow the same pattern, but in HepG2. NPM1 protein appears as the green band, Tubulin serves as a control and appears in red.
 K562_Bethyl_A302-402A_1_NPM1.png<br>Caption: IP-Western Blot analysis of K562 whole cell lysate using NPM1 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-NPM1 antibody (lanes under 'NPM1').
 NPM1_Secondary_Western.png<br>Caption: Western blot following shRNA against NPM1 in K562 and HepG2 whole cell lysate using NPM1 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against NPM1. Lanes 5-8 follow the same pattern, but in HepG2. NPM1 protein appears as the green band, Tubulin serves as a control and appears in red.
NPM1Product_ID: 325200
Lot_ID: TD268297
Source: Thermo Fisher
Target Name: NPM1-human		 NPM1-HEPG2-325200.png<br>Caption: Western blot following shRNA against NPM1 in HepG2 whole cell lysate using NPM1 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different shRNA against NPM1. NPM1 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
 ThermoFisher_325200_TD26297_NPM1.png<br>Caption: IP-WB analysis of K562 whole cell lysate using the NPM1 specific antibody, 325200. Lane 1 is 2.5% of five million whole cell lysate input. Lanes 2 and 3 are 50% of IP enrichment from five million whole cell lysate using normal IgG antibody and the NPM1-specific antibody, 325200. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
 NPM1-K562-CRISPR-325200.png<br>Caption: Western blot following CRISPR against NPM1 in K562 whole cell lysate using NPM1 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against NPM1. NPM1 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
NPM1Product_ID: 200143
Lot_ID: AD0811
Source: Zen Bioscience
Target Name: NPM1-human		 NPM1_HepG2.png<br>Caption: IP-Western Blot analysis of HepG2 whole cell lysate using NPM1 specific antibody. Lane 1 is 1.5% of ten million whole cell lysate input (lane under '5% input') , lane 2 is 30% of IP enrichment using mouse normal IgG (lane under 'IgG') and lane 3 is 30% IP enrichment using mouse monoclonal anti-NPM1 antibody (lanes under 'anti-NPM1'). Asterisk indicates light chain of antibody.