Antibodies of NCL


2 antibodies are included.     

 Released    NotSatisfied    Submitted    NotReviewed    OnHold    Submitting    Experimented   
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
NCLProduct_ID: A300-710A
Lot_ID: 1
Source: Bethyl Labs
Target Name: NCL-human		 NCL-HEPG2-CRISPR-A300-710A.png<br>Caption: Western blot following CRISPR against NCL in HepG2 whole cell lysate using NCL specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against NCL. NCL protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.
 Bethyl_A300-710A_1_NCL.png<br>Caption: IP-WB analysis of K562 whole cell lysate using the NCL specific antibody, A300-710A. Lane 1 is 2.5% of five million whole cell lysate input. Lanes 2 and 3 are 50% of IP enrichment from five million whole cell lysate using normal IgG antibody and the NCL-specific antibody, A300-710A. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
 NCL-K562-CRISPR-A300-710A.png<br>Caption: Western blot following CRISPR against NCL in K562 whole cell lysate using NCL specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against NCL. NCL protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.
NCLProduct_ID: 14574S
Lot_ID: 1
Source: Cell Signaling Technology
Target Name: NCL-human		 NCL-HEPG2-CRISPR-14574S.png<br>Caption: Western blot following CRISPR against NCL in HepG2 whole cell lysate using NCL specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against NCL. NCL protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
 CST_14574S_1_NCL.png<br>Caption: IP-WB analysis of 14574S whole cell lysate using the NCL specific antibody, 14574S. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the NCL-specific antibody, 14574S. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.