Antibodies of LIN28B


3 antibodies are included.     

 Released    NotSatisfied    Submitted    NotReviewed    OnHold    Submitting    Experimented   
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
LIN28BProduct_ID: A303-588A
Lot_ID: 1
Source: Bethyl Labs
Target Name: LIN28B-human		 HepG2_Bethyl_A303-588A_1_LIN28B.png<br>Caption: IP-Western Blot analysis of HepG2 whole cell lysate using LIN28B specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-LIN28B antibody (lanes under 'LIN28B').
 LIN28B_Secondary_Western.png<br>Caption: Western blot following shRNA against LIN28B in K562 and HepG2 whole cell lysate using LIN28B specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against LIN28B. Lanes 5-8 follow the same pattern, but in HepG2. LIN28B protein appears as the green band, Tubulin serves as a control and appears in red.
 K562_Bethyl_A303-588A_1_LIN28B.png<br>Caption: IP-Western Blot analysis of K562 whole cell lysate using LIN28B specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 10% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-LIN28B antibody (lanes under 'LIN28B').
 LIN28B_Secondary_Western.png<br>Caption: Western blot following shRNA against LIN28B in K562 and HepG2 whole cell lysate using LIN28B specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against LIN28B. Lanes 5-8 follow the same pattern, but in HepG2. LIN28B protein appears as the green band, Tubulin serves as a control and appears in red.
LIN28BProduct_ID: 11965S
Lot_ID: 1
Source: Cell Signaling Technology
Target Name: LIN28B-human		 CST_11965S_1_LIN28B.png<br>Caption: IP-WB analysis of 11965S whole cell lysate using the LIN28B specific antibody, 11965S. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the LIN28B-specific antibody, 11965S. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
LIN28BProduct_ID: 1965S
Lot_ID: 1
Source: Cell Signaling
Target Name: LIN28B-human		 lin28b-K562-CRISPR-11965S.png<br>Caption: Western blot following shRNA against LIN28B in K562 whole cell lysate using LIN28B specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNA against LIN28B. LIN28B protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.