Antibodies of EIF4G1


3 antibodies are included.     

 Released    NotSatisfied    Submitted    NotReviewed    OnHold    Submitting    Experimented   
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
EIF4G1Product_ID: MA5-14971
Lot_ID: TL2693034
Source: Thermo Fisher
Target Name: EIF4G1-human		 ThermoFisher_MA5-14971_TL2693034_EIF4G1.png<br>Caption: IP-WB analysis of K562 whole cell lysate using the EIF4G1 specific antibody, MA5-14971. Lane 1 is 2.5% of five million whole cell lysate input. Lanes 2 and 3 are 50% of IP enrichment from five million whole cell lysate using normal IgG antibody and the EIF4G1-specific antibody, MA5-14971. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
 EIF4G1-K562-MA5-14971.png<br>Caption: Western blot following shRNA against EIF4G1 in K562 whole cell lysate using EIF4G1 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNA against EIF4G1. EIF4G1 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
EIF4G1Product_ID: RN002P
Lot_ID: 2
Source: MBLI
Target Name: EIF4G1-human		 EIF4G1_Secondary_Western.png<br>Caption: Western blot following shRNA against EIF4G1 in K562 and HepG2 whole cell lysate using EIF4G1 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against EIF4G1. Lanes 5-8 follow the same pattern, but in HepG2. EIF4G1 protein appears as the green band, Tubulin serves as a control and appears in red.
 MBLI_RN002P_002_EIF4G1_K562.png<br>Caption: IP-Western Blot analysis of K562 whole cell lysate using EIF4G1 specific antibody. Lane 1 is 2.5% of 0.5mg input lysate, lane 2 is 2.5% of supernatant after immunoprecipitation and Lane 3 is 50% of IP enrichment using rabbit polyclonal Anti-EIF4G1 (Human/Mouse) pAb. This antibody passes preliminary validation and will be further pursued for primary and secondary validation.
 EIF4G1_Secondary_Western.png<br>Caption: Western blot following shRNA against EIF4G1 in K562 and HepG2 whole cell lysate using EIF4G1 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against EIF4G1. Lanes 5-8 follow the same pattern, but in HepG2. EIF4G1 protein appears as the green band, Tubulin serves as a control and appears in red.
EIF4G1Product_ID: 8701S
Lot_ID: 1
Source: Cell Signaling Technology
Target Name: EIF4G1-human		 CST_8701S_1_EIF4G1.png<br>Caption: IP-WB analysis of 8701S whole cell lysate using the EIF4G1 specific antibody, 8701S. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the EIF4G1-specific antibody, 8701S. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.