Antibodies of EIF4E


3 antibodies are included.     

 Released    NotSatisfied    Submitted    NotReviewed    OnHold    Submitting    Experimented   
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
EIF4EProduct_ID: 44-528G
Lot_ID: SG256167
Source: Thermo Fisher
Target Name: EIF4EphosphoS209-human
Phosphate: pSer209		 EIF4E-pSer209-HEPG2-CRISPR-44-528G.png<br>Caption: Western blot following CRISPR against EIF4E-pSer209 in HepG2 whole cell lysate using EIF4E-pSer209 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against EIF4E-pSer209. EIF4E-pSer209 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
 ThermoFisher_44-528G_SG256167_EIF4EpSer209.png<br>Caption: IP-WB analysis of K562 whole cell lysate using the EIF4E pSer209 specific antibody, 44-528G. Lanes 1 and 3 are 2.5% of five million whole cell lysate input. Lanes 2 and 4 are 50% of IP enrichment from five million whole cell lysate using normal IgG antibody and the EIF4E pSer209-specific antibody, 44-528G. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
 EIF4E-pSer209-k562-CRISPR-44-528G.png<br>Caption: Western blot following CRISPR against EIF4E-pSer209 in K562 whole cell lysate using EIF4E-pSer209 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against EIF4E-pSer209. EIF4E-pSer209 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
EIF4EProduct_ID: MA1-089
Lot_ID: TD257774
Source: Thermo Fisher
Target Name: EIF4E-human		 EIF4E-HEPG2-CRISPR-MA1-089.png<br>Caption: Western blot following CRISPR against EIF4E in HepG2 whole cell lysate using EIF4E specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against EIF4E. EIF4E protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
 ThermoFisher_MA1-089_TD257774_EIF4E.png<br>Caption: IP-WB analysis of K562 whole cell lysate using the EIF4E specific antibody, MA1-089. Lanes 1 and 3 are 2.5% of five million whole cell lysate input. Lanes 2 and 4 are 50% of IP enrichment from five million whole cell lysate using normal IgG antibody and the EIF4E-specific antibody, MA1-089. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
 EIF4E-K562-CRISPR-MA1-089.png<br>Caption: Western blot following CRISPR against EIF4E in K562 whole cell lysate using EIF4E specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against EIF4E. EIF4E protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
EIF4EProduct_ID: 2067S
Lot_ID: 8
Source: Cell Signaling Technology
Target Name: EIF4E-human		 CST_2067S_8_EIF4E.png<br>Caption: IP-WB analysis of 2067S whole cell lysate using the EIF4E specific antibody, 2067S. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the EIF4E-specific antibody, 2067S. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
 EIF4E-K562-CRISPR-2067s.png<br>Caption: Western blot following CRISPR against EIF4E in K562 whole cell lysate using EIF4E specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against EIF4E. EIF4E protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.