Antibodies of EIF4A3


2 antibodies are included.     

 Released    NotSatisfied    Submitted    NotReviewed    OnHold    Submitting    Experimented   
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
EIF4A3Product_ID: A302-981A
Lot_ID: 1
Source: Bethyl Labs
Target Name: EIF4A3-human		 HepG2_Bethyl_A302-981A_1_EIF4A3.png<br>Caption: IP-Western Blot analysis of HepG2 whole cell lysate using EIF4A3 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-EIF4A3 antibody (lanes under 'EIF4A3').
 EIF4A3_Secondary_Western.png<br>Caption: Western blot following shRNA against EIF4A3 in K562 and HepG2 whole cell lysate using EIF4A3 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 2 and 3 are two different shRNAs against EIF4A3. Lanes 5-7 follow the same pattern, but in HepG2. EIF4A3 protein appears as the green band, GAPDH serves as a control and appears in red.
 K562_Bethyl_A302-981A_1_EIF4A3.png<br>Caption: IP-Western Blot analysis of K562 whole cell lysate using EIF4A3 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-EIF4A3 antibody (lanes under 'EIF4A3').
 EIF4A3_Secondary_Western.png<br>Caption: Western blot following shRNA against EIF4A3 in K562 and HepG2 whole cell lysate using EIF4A3 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 2 and 3 are two different shRNAs against EIF4A3. Lanes 5-7 follow the same pattern, but in HepG2. EIF4A3 protein appears as the green band, GAPDH serves as a control and appears in red.
EIF4A3Product_ID: PA5-54532
Lot_ID: TK2671524A
Source: Thermo Fisher
Target Name: EIF4A3-human		 EIF4A3-HEPG2-CRISPR-PA5-54532.png<br>Caption: Western blot following CRISPR against EIF4A3 in HepG2 whole cell lysate using EIF4A3 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against EIF4A3. EIF4A3 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
 ThermoFisher_PA5-54532_TK2671524A_EIF4A3.png<br>Caption: IP-WB analysis of K562 whole cell lysate using the EIF4A3 specific antibody, PA5-54532. Lanes 1 and 3 are 2.5% of five million whole cell lysate input. Lanes 2 and 4 are 50% of IP enrichment from five million whole cell lysate using normal IgG antibody and the EIF4A3-specific antibody, PA5-54532. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
 EIF4A3-K562-CRISPR-PA5-54532.png<br>Caption: Western blot following CRISPR against EIF4A3 in K562 whole cell lysate using EIF4A3 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against EIF4A3. EIF4A3 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.