Antibodies of EIF2S1


3 antibodies are included.     

 Released    NotSatisfied    Submitted    NotReviewed    OnHold    Submitting    Experimented   
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
EIF2S1Product_ID: A300-721A
Lot_ID: 1
Source: Bethyl Labs
Target Name: EIF2S1-human		 HepG2_Bethyl_A300-721A_1_EIF2S1.png<br>Caption: IP-Western Blot analysis of HepG2 whole cell lysate using EIF2S1 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-EIF2S1 antibody (lanes under 'EIF2S1').
 EIF2S1_Secondary_Western.png<br>Caption: Western blot following shRNA against EIF2S1 in K562 and HepG2 whole cell lysate using EIF2S1 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 2 and 3 are two different shRNAs against EIF2S1. Lanes 5-7 follow the same pattern, but in HepG2. EIF2S1 protein appears as the green band, GAPDH serves as a control and appears in red.
 K562_Bethyl_A300-721A_1_EIF2S1.png<br>Caption: IP-Western Blot analysis of K562 whole cell lysate using EIF2S1 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-EIF2S1 antibody (lanes under 'EIF2S1').
 EIF2S1_Secondary_Western.png<br>Caption: Western blot following shRNA against EIF2S1 in K562 and HepG2 whole cell lysate using EIF2S1 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 2 and 3 are two different shRNAs against EIF2S1. Lanes 5-7 follow the same pattern, but in HepG2. EIF2S1 protein appears as the green band, GAPDH serves as a control and appears in red.
EIF2S1Product_ID: AHO0802
Lot_ID: TC262147
Source: Thermo Fisher
Target Name: EIF2S1-human		 ThermoFisher_AHO0802_TC262147_EIF2S1.png<br>Caption: IP-WB analysis of K562 whole cell lysate using the EIF2S1 specific antibody, AHO0802. Lanes 1 and 3 are 2.5% of five million whole cell lysate input. Lanes 2 and 4 are 50% of IP enrichment from five million whole cell lysate using normal IgG antibody and the EIF2S1-specific antibody, AHO0802. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
EIF2S1Product_ID: 5324S
Lot_ID: 9
Source: Cell Signaling Technology
Target Name: EIF2S1-human		 CST_5324S_9_EIF2S1.png<br>Caption: IP-WB analysis of 5324S whole cell lysate using the EIF2S1 specific antibody, 5324S. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the EIF2S1-specific antibody, 5324S. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
 EIF2S1-K562-CRISPR-5324S.png<br>Caption: Western blot following CRISPR against EIF2S1 in K562 whole cell lysate using EIF2S1 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against EIF2S1. EIF2S1 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.