Antibodies of E4F1


1 antibodies are included.     

 Released    NotSatisfied    Submitted    NotReviewed    OnHold    Submitting    Experimented   
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
E4F1Product_ID: PA5-67167
Lot_ID: UC2739267A
Source: Thermo Fisher
Target Name: E4F1-human
E4F1-HEPG2-CRISPR-PA5-67167.png<br>Caption: Western blot following CRISPR against E4F1 in HepG2 whole cell lysate using E4F1 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against E4F1. E4F1 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.
ThermoFisher_PA5-67167_UC2739267A_E4F1.png<br>Caption: IP-WB analysis of K562 whole cell lysate using the E4F1 specific antibody, PA5-67167. Lane 1 is 2.5% of five million whole cell lysate input. Lanes 2 and 3 are 50% of IP enrichment from five million whole cell lysate using normal IgG antibody and the E4F1-specific antibody, PA5-67167. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
E4F1-K562-CRISPR-PA5-67167.png<br>Caption: Western blot following CRISPR against E4F1 in K562 whole cell lysate using E4F1 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against E4F1. E4F1 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.