Antibodies of DDX19B


3 antibodies are included.     

 Released    NotSatisfied    Submitted    NotReviewed    OnHold    Submitting    Experimented   
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
DDX19BProduct_ID: A300-545A
Lot_ID: 1
Source: Bethyl Labs
Target Name: DDX19B-human		 HepG2_Bethyl_A300-545A_1_DDX19B.png<br>Caption: IP-Western Blot analysis of HepG2 whole cell lysate using DDX19B specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-DDX19B antibody (lanes under 'DDX19B').
 DDX19B_Secondary_Western.png<br>Caption: Western blot following shRNA against DDX19B in K562 and HepG2 whole cell lysate using DDX19B specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against DDX19B. Lanes 5-8 follow the same pattern, but in HepG2. DDX19B protein appears as the green band, Tubulin serves as a control and appears in red.
 K562_Bethyl_A300-545A_1_DDX19B.png<br>Caption: IP-Western Blot analysis of K562 whole cell lysate using DDX19B specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-DDX19B antibody (lanes under 'DDX19B').
 DDX19B_Secondary_Western.png<br>Caption: Western blot following shRNA against DDX19B in K562 and HepG2 whole cell lysate using DDX19B specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against DDX19B. Lanes 5-8 follow the same pattern, but in HepG2. DDX19B protein appears as the green band, Tubulin serves as a control and appears in red.
DDX19BProduct_ID: A300-546A
Lot_ID: 1
Source: Bethyl Labs
Target Name: DDX19B-human		 Bethyl_A300-546A_1_DDX19B.png<br>Caption: IP-WB analysis of K562 whole cell lysate using the DDX19B specific antibody, A300-546A. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the DDX19B-specific antibody, A300-546A. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
 DDX19B-K562-A300-546A.png<br>Caption: Western blot following shRNA against DDX19B in K562 whole cell lysate using DDX19B specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNA against DDX19B. DDX19B protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
DDX19BProduct_ID: A300-547A
Lot_ID: 1
Source: Bethyl Labs
Target Name: DDX19B-human		 Bethyl_A300-547A_1_DDX19B.png<br>Caption: IP-WB analysis of K562 whole cell lysate using the DDX19B specific antibody, A300-547A. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the DDX19B-specific antibody, A300-547A. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.