Antibodies of CTCF


2 antibodies are included.     

 Released    NotSatisfied    Submitted    NotReviewed    OnHold    Submitting    Experimented   
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
CTCFProduct_ID: PA5-17143
Lot_ID: UC2731408
Source: Thermo Fisher
Target Name: CTCF-human		 CTCF-HepG2-CRISPR-PA5-17143.png<br>Caption: Western blot following CRISPR against CTCF in HepG2 whole cell lysate using CTCF specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against CTCF. CTCF protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.
 ThermoFisher_PA5-17143_UC2731408_CTCF.png<br>Caption: IP-WB analysis of K562 whole cell lysate using the CTCF specific antibody, PA5-17143. Lane 1 is 2.5% of five million whole cell lysate input. Lanes 2 and 3 are 50% of IP enrichment from five million whole cell lysate using normal IgG antibody and the CTCF-specific antibody, PA5-17143. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
 CTCF-K562-CRISPR-PA5-17143.png<br>Caption: Western blot following CRISPR against CTCF in K562 whole cell lysate using CTCF specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against CTCF. CTCF protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.
CTCFProduct_ID: 3418S
Lot_ID: 5
Source: Cell Signaling Technology
Target Name: CTCF-human		 CTCF-HEPG2-CRISPR-3418S.png<br>Caption: Western blot following CRISPR against CTCF in HepG2 whole cell lysate using CTCF specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against CTCF. CTCF protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
 CST_3418S_5_CTCF.png<br>Caption: IP-WB analysis of 3418S whole cell lysate using the CTCF specific antibody, 3418S. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the CTCF-specific antibody, 3418S. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.