Antibodies of CDK9


2 antibodies are included.     

 Released    NotSatisfied    Submitted    NotReviewed    OnHold    Submitting    Experimented   
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
CDK9Product_ID: A303-493A
Lot_ID: 1
Source: Bethyl Labs
Target Name: CDK9-human		 CDK9-HEPG2-CRISPR-A303-493A.png<br>Caption: Western blot following CRISPR against CDK9 in HepG2 whole cell lysate using CDK9 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against CDK9. CDK9 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
 Bethyl_A303-493A_1_CDK9.png<br>Caption: IP-WB analysis of K562 whole cell lysate using the CDK9 specific antibody, A303-493A. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the CDK9-specific antibody, A303-493A. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
 CDK9-K562-CRISPR-A303-493A.png<br>Caption: Western blot following CRISPR against CDK9 in K562 whole cell lysate using CDK9 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against CDK9. CDK9 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.
CDK9Product_ID: 2316S
Lot_ID: 9
Source: Cell Signaling Technology
Target Name: CDK9-human		 CDK9-HEPG2-CRISPR-2316S.png<br>Caption: Western blot following CRISPR against CDK9 in HepG2 whole cell lysate using CDK9 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against CDK9. CDK9 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
 CST_2316S_9_CDK9.png<br>Caption: IP-WB analysis of 2316S whole cell lysate using the CDK9 specific antibody, 2316S. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the CDK9-specific antibody, 2316S. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.