Antibodies of CAST


3 antibodies are included.     

 Released    NotSatisfied    Submitted    NotReviewed    OnHold    Submitting    Experimented   
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
CASTProduct_ID: A301-295A
Lot_ID: 1
Source: Bethyl Labs
Target Name: CAST-human		 Bethyl_A301-295A_1_CD3EAP.png<br>Caption: IP-WB analysis of K562 whole cell lysate using the CD3EAP specific antibody, A301-295A. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the CD3EAP-specific antibody, A301-295A. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
CASTProduct_ID: A304-793A
Lot_ID: 1
Source: Bethyl Labs
Target Name: CAST-human		 Bethyl_A304-793A_1_CAST.png<br>Caption: IP-WB analysis of K562 whole cell lysate using the CAST specific antibody, A304-793A. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the CAST-specific antibody, A304-793A. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
CASTProduct_ID: A304-794A
Lot_ID: 1
Source: Bethyl Labs
Target Name: CAST-human		 Bethyl_A304-794A_1_CAST.png<br>Caption: IP-WB analysis of K562 whole cell lysate using the CAST specific antibody, A304-794A. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the CAST-specific antibody, A304-794A. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
 CAST-K562-CRISPR-A304-794A.png<br>Caption: Western blot following CRISPR against CAST in K562 whole cell lysate using CAST specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against CAST. CAST protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.