Antibodies of BRD4


2 antibodies are included.     

 Released    NotSatisfied    Submitted    NotReviewed    OnHold    Submitting    Experimented   
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
BRD4Product_ID: 13440S
Lot_ID: 9
Source: Cell Signaling Technology
Target Name: BRD4-human		 CST_13440S_9_BRD4.png<br>Caption: IP-WB analysis of 13440S whole cell lysate using the BRD4 specific antibody, 13440S. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the BRD4-specific antibody, 13440S. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
 BRD4-K562-CRISPR-13440S.png<br>Caption: Western blot following CRISPR against BRD4 in K562 whole cell lysate using BRD4 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against BRD4. BRD4 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
BRD4Product_ID: 83375S
Lot_ID: 1
Source: Cell Signaling Technology
Target Name: BRD4-human		 CST_83375S_1_BRD4.png<br>Caption: IP-WB analysis of 83375S whole cell lysate using the BRD4 specific antibody, 83375S. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the BRD4-specific antibody, 83375S. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.