Antibodies of ARHGEF1


3 antibodies are included.     

 Released    NotSatisfied    Submitted    NotReviewed    OnHold    Submitting    Experimented   
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
ARHGEF1Product_ID: A302-701A
Lot_ID: 1
Source: Bethyl Labs
Target Name: ARHGEF1-human
ARHGEF1-HEPG2-CRISPR-A302-701A.png<br>Caption: Western blot following CRISPR against ARHGEF1 in HepG2 whole cell lysate using ARHGEF1 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against ARHGEF1. ARHGEF1 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.
Bethyl_A302-701A_1_ARHGEF1.png<br>Caption: IP-WB analysis of K562 whole cell lysate using the ARHGEF1 specific antibody, A302-701A. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the ARHGEF1-specific antibody, A302-701A. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
ARHGEF1-K562-CRISPR-A302-701A.png<br>Caption: Western blot following CRISPR against ARHGEF1 in K562 whole cell lysate using ARHGEF1 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against ARHGEF1. ARHGEF1 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
ARHGEF1Product_ID: A302-702A
Lot_ID: 1
Source: Bethyl Labs
Target Name: ARHGEF1-human
Bethyl_A302-702A_1_ARHGEF1.png<br>Caption: IP-WB analysis of K562 whole cell lysate using the ARHGEF1 specific antibody, A302-702A. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the ARHGEF1-specific antibody, A302-702A. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
ARHGEF1-K562-CRISPR-A302-702A.png<br>Caption: Western blot following CRISPR against ARHGEF1 in K562 whole cell lysate using ARHGEF1 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against ARHGEF1. ARHGEF1 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
ARHGEF1Product_ID: 3669S
Lot_ID: 3
Source: Cell Signaling Technology
Target Name: ARHGEF1-human
CST_3669S_3_ARHGEF1.png<br>Caption: IP-WB analysis of 3669S whole cell lysate using the ARHGEF1 specific antibody, 3669S. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the ARHGEF1-specific antibody, 3669S. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
ARHGEF1-K562-CRISPR-3669S.png<br>Caption: Western blot following CRISPR against ARHGEF1 in K562 whole cell lysate using ARHGEF1 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against ARHGEF1. ARHGEF1 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.