Antibodies of API5


1 antibodies are included.     

 Released    NotSatisfied    Submitted    NotReviewed    OnHold    Submitting    Experimented   
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
API5Product_ID: A302-784A
Lot_ID: 2
Source: Bethyl Labs
Target Name: API5-human		 API5-HEPG2-CRISPR-A302-784A.png<br>Caption: Western blot following CRISPR against API5 in HepG2 whole cell lysate using API5 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against API5. API5 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.
 Bethyl_A302-784A_2_API5.png<br>Caption: IP-WB analysis of K562 whole cell lysate using the API5 specific antibody, A302-784A. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the API5-specific antibody, A302-784A. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
 API5-K562-CRISPR-A302-784A.png<br>Caption: Western blot following CRISPR against API5 in K562 whole cell lysate using API5 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against API5. API5 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.