Antibodies of ALYREF


1 antibodies are included.     

 Released    NotSatisfied    Submitted    NotReviewed    OnHold    Submitting    Experimented   
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
ALYREFProduct_ID: 12655S
Lot_ID: 1
Source: Cell Signaling Technology
Target Name: ALYREF-human		 ALYREF-HEPG2-CRISPR-12655S.png<br>Caption: Western blot following CRISPR against ALYREF in HepG2 whole cell lysate using ALYREF specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against ALYREF. ALYREF protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
 CST_12655S_1_ALYREF.png<br>Caption: IP-WB analysis of 12655S whole cell lysate using the ALYREF specific antibody, 12655S. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the ALYREF-specific antibody, 12655S. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
 ALYREF-K562-CRISPR-12655S.png<br>Caption: Western blot following CRISPR against ALYREF in K562 whole cell lysate using ALYREF specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against ALYREF. ALYREF protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.