Antibodies of ADAR


2 antibodies are included.     

 Released    NotSatisfied    Submitted    NotReviewed    OnHold    Submitting    Experimented   
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
ADARProduct_ID: A303-883A
Lot_ID: 1
Source: Bethyl Labs
Target Name: ADAR-human		 ADAR1_Secondary_Western.png<br>Caption: Western blot following shRNA against ADAR1 in K562 and HepG2 whole cell lysate using ADAR1 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against ADAR1. Lanes 5-8 follow the same pattern, but in HepG2. ADAR1 protein appears as the green band, Tubulin serves as a control and appears in red.
 Bethyl_A303-883A_3_ADAR.png<br>Caption: IP-WB analysis of K562 whole cell lysate using the ADAR specific antibody, A303-883A. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the ADAR-specific antibody, A303-883A. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
K562_Bethyl_A303-883A_1_ADAR1.png<br>Caption: IP-Western Blot analysis of K562 whole cell lysate using ADAR1 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 10% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-ADAR1 antibody (lanes under 'ADAR1').
 ADAR1_Secondary_Western.png<br>Caption: Western blot following shRNA against ADAR1 in K562 and HepG2 whole cell lysate using ADAR1 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against ADAR1. Lanes 5-8 follow the same pattern, but in HepG2. ADAR1 protein appears as the green band, Tubulin serves as a control and appears in red.
ADAR-K562-A303-883A.png<br>Caption: Western blot following shRNA against ADAR in K562 whole cell lysate using ADAR specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNA against ADAR. ADAR protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
ADARProduct_ID: 81284S
Lot_ID: 1
Source: Cell Signaling Technology
Target Name: ADAR-human		 CST_81284S_1_ADAR.png<br>Caption: IP-WB analysis of 81284S whole cell lysate using the ADAR specific antibody, 81284S. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the ADAR-specific antibody, 81284S. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
 ADAR-K562-81284S.png<br>Caption: Western blot following shRNA against ADAR in K562 whole cell lysate using ADAR specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNA against ADAR. ADAR protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.