RPL18      CRISPR in K562      Batch: BGKcLV53

Experiment Information (Status: LibPreping)
BGKcLV53-103BGKcLV53-104
idx00
TRCN#_or_BGC#BGC#0000621BGC#0000621
shRNA_or_gRNA_sequenceCCTTCCCTAGGTCCTCGCAACCTTCCCTAGGTCCTCGCAA
PAMGGGGGG
NameRPL18_88FRPL18_88F
Sample_IDBGKcLV53-103BGKcLV53-104
transduction_Date2/24/262/24/26
daysD6D6
RBP_nameRPL18RPL18
qPCR_result0.00.3
Ave_qPCR
RT-qPCR_primer-F
RT-qPCR_primer-R
protein_conc28872407
WB_result65.750.2021
Ave_WB
WB_DONE_date3/31/26
MW22kd
IP
antibody_Cat#A305-340Alot #1
Antibody DCC ID
submitted_to_DCC_date
Rep1_TPM00
Rep2_TPM00
ActionReadyReady
Library_start_date
repeat_library
Note
ID64656466




Western Blot
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
RPL18Product_ID: A305-340A
Lot_ID: 1
Source: Bethyl Labs
Target Name: RPL18-human		 RPL18-HEPG2-CRISPR-A305-340A.png<br>Caption: Western blot following CRISPR against RPL18 in HepG2 whole cell lysate using RPL18 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against RPL18. RPL18 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
 RPL18-K562-CRISPR-A305-340A.png<br>Caption: Western blot following CRISPR against RPL18 in K562 whole cell lysate using RPL18 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against RPL18. RPL18 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.




Experiment Status
BGKcLV53-103BGKcLV53-104
Sample_IDBGKcLV53-103BGKcLV53-104
Sample Name
Sample Name
RBPRPL18RPL18
Cell_LineK562K562
Exp UID
StatusLibPrepingLibPreping
Status_date2026-05-182026-05-18
ProjectENCORE2ENCORE2
Note
ID1480214803




Library-Prep Information
BGKcLV53-103BGKcLV53-104
Sample #5354
Sample NameRPL18-BGKcLV53-103RPL18-BGKcLV53-104
Sample_Name_Alias
Index Well PositionC08D08
Index_tableIDT_UniqueDualIndex_96_V2IDT_UniqueDualIndex_96_V2
LibPrep_date2026-05-262026-05-26
Lib_IDLib-260526Lib-260526
Tecan_Location
Tecan
Tecan_date
Size_bp282286
Peak_Molarity68.7073.20
libSampleQC_DNA_Well
RIN
libSample_RNA_Well
SampleQC_methodTapeStation_2022TapeStation_2022
SampleQC_date2026-05-272026-05-27
Sample_IDBGKcLV53-103BGKcLV53-104
RBPRPL18RPL18
Batch_IDBGKcLV53BGKcLV53
WB_result65.70050.202
Library DescriptionTruSeq mRNATruSeq mRNA
Repeat_Library_Suffix
Lib_StatusSampleQCedSampleQCed
ProjectENCORE2ENCORE2
ID48924893




Sequencing Information
Sequencing info was not avaliable




Data Submission Information
DCC submission information was not avaliable




File Information
Files of the experiment was not avaliable in the database