NEDD4      CRISPR in K562      Batch: BGKcLV51

Experiment Information (Status: LibPreping)
BGKcLV51-71BGKcLV51-72
idx00
TRCN#_or_BGC#BGC#0001475BGC#0001475
shRNA_or_gRNA_sequenceGGAAATGGCAACTTGCGCGGGGAAATGGCAACTTGCGCGG
PAMTGGTGG
NameNEDD4_98NEDD4_98
Sample_IDBGKcLV51-71BGKcLV51-72
transduction_Date9/30/20259/30/2025
daysD6D6
RBP_nameNEDD4NEDD4
qPCR_result8.70.8
Ave_qPCR4.8
RT-qPCR_primer-Fgagctgaaaaatggtggatca
RT-qPCR_primer-Rcagccatttgcttctggatt
protein_conc24522885
WB_result68.869.8
Ave_WB
WB_DONE_date10/28/25
MW149kd
IP
antibody_Cat#A303-254A lot# 1
Antibody DCC ID
submitted_to_DCC_date
Rep1_TPM00
Rep2_TPM00
ActionReadyReady
Library_start_date
repeat_library
Note
ID63126313




Western Blot
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
NEDD4Product_ID: A303-254
Lot_ID: 1
Source: Bethyl Labs
Target Name: NEDD4-human		 NEDD4-K562-CRISPR-A303-254A.png<br>Caption: Western blot following CRISPR against NEDD4 in K562 whole cell lysate using NEDD4 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against NEDD4. NEDD4 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.




Experiment Status
BGKcLV51-71BGKcLV51-72
Sample_IDBGKcLV51-71BGKcLV51-72
Sample Name
Sample Name
RBPNEDD4NEDD4
Cell_LineK562K562
Exp UID
StatusLibPrepingLibPreping
Status_date2026-05-132026-05-13
ProjectENCORE2ENCORE2
Note
ID1457614577




Library-Prep Information
BGKcLV51-71BGKcLV51-72
Sample #1112
Sample NameNEDD4-BGKcLV51-71NEDD4-BGKcLV51-72
Sample_Name_Alias
Index Well PositionC02D02
Index_tableIDT_UniqueDualIndex_96_V2IDT_UniqueDualIndex_96_V2
LibPrep_date2026-05-182026-05-18
Lib_IDLib-260518Lib-260518
Tecan_Location
Tecan
Tecan_date
Size_bp300303
Peak_Molarity54.5087.50
libSampleQC_DNA_Well
RIN
libSample_RNA_Well
SampleQC_methodTapeStation_2022TapeStation_2022
SampleQC_date2026-05-212026-05-21
Sample_IDBGKcLV51-71BGKcLV51-72
RBPNEDD4NEDD4
Batch_IDBGKcLV51BGKcLV51
WB_result68.80069.800
Library DescriptionTruSeq mRNATruSeq mRNA
Repeat_Library_Suffix
Lib_StatusSampleQCedSampleQCed
ProjectENCORE2ENCORE2
ID48504851




Sequencing Information
Sequencing info was not avaliable




Data Submission Information
DCC submission information was not avaliable




File Information
Files of the experiment was not avaliable in the database