ATXN2      CRISPR in K562      Batch: BGKcLV51

Experiment Information (Status: LibPreping)
BGKcLV51-17BGKcLV51-18
idx00
TRCN#_or_BGC#BGC#0001453BGC#0001453
shRNA_or_gRNA_sequenceGTCGCCGCGACCACCGAGGGTCGCCGCGACCACCGAGG
PAMAGGAGG
NameATX2_88/ATXN2ATX2_88/ATXN2
Sample_IDBGKcLV51-17BGKcLV51-18
transduction_Date9/30/20259/30/2025
daysD6D6
RBP_nameATXN2ATXN2
qPCR_result48.632.1
Ave_qPCR40.4
RT-qPCR_primer-Fttcaacccacgttccttctc
RT-qPCR_primer-Rgctgttgatgacccaccata
protein_conc23581959
WB_result57.562.3
Ave_WB
WB_DONE_date10/9/25
MW140KD
IP
antibody_Cat#A301-118Aold
Antibody DCC IDENCAB360GKL
submitted_to_DCC_date
Rep1_TPM00
Rep2_TPM00
ActionReadyReady
Library_start_date
repeat_library
Note
ID62546255




Western Blot
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
ATXN2Product_ID: A301-118A
Lot_ID: 2
Source: Bethyl Labs
Target Name: ATXN2-human		 Bethyl_A301-118A_2_ATXN2.png<br>Caption: IP-WB analysis of K562 whole cell lysate using the ATXN2 specific antibody, A301-118A. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the ATXN2-specific antibody, A301-118A. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
 ATXN2-K562-CRISPR-A301-118A.png<br>Caption: Western blot following CRISPR against ATXN2 in K562 whole cell lysate using ATXN2 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against ATXN2. ATXN2 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.




Experiment Status
BGKcLV51-17BGKcLV51-18
Sample_IDBGKcLV51-17BGKcLV51-18
Sample Name
Sample Name
RBPATXN2ATXN2
Cell_LineK562K562
Exp UID
StatusLibPrepingLibPreping
Status_date2026-05-132026-05-13
ProjectENCORE2ENCORE2
Note
ID1456814569




Library-Prep Information
BGKcLV51-17BGKcLV51-18
Sample #34
Sample NameATXN2-BGKcLV51-17ATXN2-BGKcLV51-18
Sample_Name_Alias
Index Well PositionC01D01
Index_tableIDT_UniqueDualIndex_96_V2IDT_UniqueDualIndex_96_V2
LibPrep_date2026-05-182026-05-18
Lib_IDLib-260518Lib-260518
Tecan_Location
Tecan
Tecan_date
Size_bp289299
Peak_Molarity45.0080.80
libSampleQC_DNA_Well
RIN
libSample_RNA_Well
SampleQC_methodTapeStation_2022TapeStation_2022
SampleQC_date2026-05-212026-05-21
Sample_IDBGKcLV51-17BGKcLV51-18
RBPATXN2ATXN2
Batch_IDBGKcLV51BGKcLV51
WB_result57.50062.300
Library DescriptionTruSeq mRNATruSeq mRNA
Repeat_Library_Suffix
Lib_StatusSampleQCedSampleQCed
ProjectENCORE2ENCORE2
ID48424843




Sequencing Information
Sequencing info was not avaliable




Data Submission Information
DCC submission information was not avaliable




File Information
Files of the experiment was not avaliable in the database