SYNCRIP      CRISPR in HepG2      Batch: BGHcLV47

Experiment Information (Status: Sequenced)
BGHcLV47-79BGHcLV47-79BGHcLV47-80BGHcLV47-80
idx0000
TRCN#_or_BGC#BGC#0000104BGC#0000104BGC#0000104BGC#0000104
shRNA_or_gRNA_sequenceGTGGAGTCATGAAGACTTACGTGGAGTCATGAAGACTTACGTGGAGTCATGAAGACTTACGTGGAGTCATGAAGACTTAC
PAMAGGAGGAGGAGG
NameSYNCRIP-73-1SYNCRIP-73-1SYNCRIP-73-1SYNCRIP-73-1
Sample_IDBGHcLV47-79BGHcLV47-79BGHcLV47-80BGHcLV47-80
transduction_Date1/28/251/28/251/28/251/28/25
daysD6D6D6D6
RBP_nameSYNCRIPSYNCRIPSYNCRIPSYNCRIP
qPCR_result62.762.760.560.5
Ave_qPCR
RT-qPCR_primer-F
RT-qPCR_primer-R
protein_conc4616461652285228
WB_result77.263.977.669.5
Ave_WB
WB_DONE_date2/6/253/13/26
MW70kD70kD
IPfu's
antibody_Cat#RN046PW8588SLOT# 1
Antibody DCC IDENCAB548DAU
submitted_to_DCC_date
Rep1_TPM0000
Rep2_TPM0000
ActionReadyReady
Library_start_date
repeat_library
Note
ID9868987098699871




Western Blot
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
SYNCRIPProduct_ID: RN046PW
Lot_ID: 001
Source: MBLI
Target Name: SYNCRIP-human		 SYNCRIP-HEPG2-CRISPR-RN046PW.png<br>Caption: Western blot following CRISPR against SYNCRIP in HepG2 whole cell lysate using SYNCRIP specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against SYNCRIP. SYNCRIP protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
SYNCRIPProduct_ID: 8588S
Lot_ID: 1
Source: Cell Signaling Technology
Target Name: SYNCRIP-human		 SYNCRIP-HEPG2-CRISPR-8588S.png<br>Caption: Western blot following CRISPR against SYNCRIP in HepG2 whole cell lysate using SYNCRIP specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against SYNCRIP. SYNCRIP protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
 CST_8588S_1_HNRNPQ.png<br>Caption: IP-WB analysis of 8588S whole cell lysate using the HNRNPQ/HNRNPR specific antibody, 8588S. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the HNRNPQ/HNRNPR-specific antibody, 8588S. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.




Experiment Status
BGHcLV47-79BGHcLV47-80
Sample_IDBGHcLV47-79BGHcLV47-80
Sample Name
Sample NameSYNCRIP-BGHcLV47-79SYNCRIP-BGHcLV47-80
RBPSYNCRIPSYNCRIP
Cell_LineHepG2HepG2
Exp UID
StatusSequencedSequenced
Status_date2025-05-142025-05-14
ProjectENCORE2ENCORE2
Note
ID1417614177




Library-Prep Information
BGHcLV47-79BGHcLV47-80
Sample #2930
Sample NameSYNCRIP-BGHcLV47-79SYNCRIP-BGHcLV47-80
Sample_Name_Alias
Index Well PositionE11F11
Index_tableIDT_UniqueDualIndex_96_V2IDT_UniqueDualIndex_96_V2
LibPrep_date2025-04-232025-04-23
Lib_IDLib-250423Lib-250423
Tecan_Location
Tecan
Tecan_date
Size_bp273268
Peak_Molarity92.4085.20
libSampleQC_DNA_Well
RIN
libSample_RNA_Well
SampleQC_methodTapeStation_2022TapeStation_2022
SampleQC_date2025-04-222025-04-22
Sample_IDBGHcLV47-79BGHcLV47-80
RBPSYNCRIPSYNCRIP
Batch_IDBGHcLV47BGHcLV47
WB_result77.20077.600
Library DescriptionTruSeq mRNATruSeq mRNA
Repeat_Library_Suffix
Lib_StatusSendToSequenceSendToSequence
ProjectENCORE2ENCORE2
ID47404741




Sequencing Information
BGHcLV47-79BGHcLV47-80
Sample_IDBGHcLV47-79BGHcLV47-80
Sample NameSYNCRIP-BGHcLV47-79SYNCRIP-BGHcLV47-80
Pool IDPool-250422Pool-250422
LocalServer_folder
total_reads00
total_aligned_reads00
unique_aligned_reads00
percent_uniqueAligned
correlation_replicates
spikein_reads00
percent_spikeins
original_ReadLength
QC_StatusReadyToQCReadyToQC
ID34433444




Data Submission Information
DCC submission information was not avaliable




File Information
Files of the experiment was not avaliable in the database