HNRNPR antibody: 8588S

Caption: Western blot following CRISPR against SYNCRIP in HepG2 whole cell lysate using SYNCRIP specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against SYNCRIP. SYNCRIP protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.

Releated Sample: BGHcLV47-79

Status: Experimented

Lab: Graveley Lab

Caption: IP-WB analysis of 8588S whole cell lysate using the HNRNPQ/HNRNPR specific antibody, 8588S. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the HNRNPQ/HNRNPR-specific antibody, 8588S. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.

Method: immunoprecipitation

Status: Submitted

Lab: Yeo Lab

Caption: IP-WB analysis of 8588S whole cell lysate using the HNRNPQ/HNRNPR specific antibody, 8588S. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the HNRNPQ/HNRNPR-specific antibody, 8588S. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.

Method: immunoprecipitation

Status: Submitted

Lab: Yeo Lab