non-target      CRISPR in HepG2      Control: NT-BGHcLV45-1,NT-BGHcLV45-2

General Information

List KD experiments controlled by BGHcLV45-1 and BGHcLV45-2


NT-BGHcLV45-1,NT-BGHcLV45-2:
  • ADD1-BGHcLV45-HepG2
  • ALYREF-BGHcLV45-HepG2
  • CAB39-BGHcLV45-HepG2
  • CCNT1-BGHcLV45-HepG2
  • CDK11B-BGHcLV45-HepG2
  • DCP1B-BGHcLV45-HepG2
  • DEK-BGHcLV45-HepG2
  • EED-BGHcLV45-HepG2
  • EIF5B-BGHcLV45-HepG2
  • GFPT1-BGHcLV45-HepG2
  • RANGAP1-BGHcLV45-HepG2
  • RBMX-BGHcLV45-HepG2
  • SENP3-BGHcLV45-HepG2
  • SRRT-BGHcLV45-HepG2
  • SRSF10-BGHcLV45-HepG2
  • SSR1-BGHcLV45-HepG2
  • SYMPK-BGHcLV45-HepG2
  • TFRC-BGHcLV45-HepG2
  • YARS-BGHcLV45-HepG2
  • YTHDF2-BGHcLV45-HepG2




    • Experiment Information (Status: Submitting)
    BGHcLV45-1BGHcLV45-2
    idx14341435
    TRCN#_or_BGC#
    shRNA_or_gRNA_sequence
    PAM
    Namenonnon
    Sample_IDBGHcLV45-1BGHcLV45-2
    transduction_Date10/1/2410/1/24
    daysD6D6
    RBP_namenon-targetnon-target
    qPCR_result
    Ave_qPCR
    RT-qPCR_primer-F
    RT-qPCR_primer-R
    protein_conc04508
    WB_result
    Ave_WB
    WB_DONE_date
    MW
    IP
    antibody_Cat#
    Antibody DCC ID
    submitted_to_DCC_date
    Rep1_TPM00
    Rep2_TPM00
    ActionReadyReady
    Library_start_date
    repeat_library
    Note
    ID96899690




    Western Blot
    Western Blot info was not avaliable




    Experiment Status
    BGHcLV45-1BGHcLV45-2
    Sample_IDBGHcLV45-1BGHcLV45-2
    Sample Name
    Sample NameNT-BGHcLV45-1NT-BGHcLV45-2
    RBPnon-targetnon-target
    Cell_LineHepG2HepG2
    Exp UID
    StatusSubmittingSubmitting
    Status_date2025-04-212025-04-21
    ProjectENCORE2ENCORE2
    Note
    ID1368613687




    Library-Prep Information
    BGHcLV45-1BGHcLV45-2
    Sample #12
    Sample NameNT-BGHcLV45-1NT-BGHcLV45-2
    Sample_Name_Alias
    Index Well PositionA07B07
    Index_tableIDT_UniqueDualIndex_96_V2IDT_UniqueDualIndex_96_V2
    LibPrep_date2024-12-022024-12-02
    Lib_IDLib-241202Lib-241202
    Tecan_Location
    Tecan
    Tecan_date
    Size_bp289282
    Peak_Molarity72.1068.60
    libSampleQC_DNA_WellDNA_Library_set76/set1/A7.pngDNA_Library_set76/set1/B7.png
    RIN10.010.0
    libSample_RNA_WellRNA_Library_set76/set1/A1.pngRNA_Library_set76/set1/B1.png
    SampleQC_methodTapeStation_2022TapeStation_2022
    SampleQC_date2024-12-052024-12-05
    Sample_IDBGHcLV45-1BGHcLV45-2
    RBPNTNT
    Batch_IDBGHcLV45BGHcLV45
    WB_result0.0000.000
    Library DescriptionTruSeq mRNATruSeq mRNA
    Repeat_Library_Suffix
    Lib_StatusSendToSequenceSendToSequence
    ProjectENCORE2ENCORE2
    ID46104611




    Sequencing Information
    BGHcLV45-1BGHcLV45-2
    Sample_IDBGHcLV45-1BGHcLV45-2
    Sample NameNT-BGHcLV45-1NT-BGHcLV45-2
    Pool IDPool-241205Pool-241205
    LocalServer_folderset76set76
    total_reads26,992,13523,947,697
    total_aligned_reads22,487,05119,553,077
    unique_aligned_reads20,322,84418,352,090
    percent_uniqueAligned0.752920.76634
    correlation_replicates0.9931320.993132
    spikein_reads44,15235,504
    percent_spikeins0.001640.00148
    original_ReadLength101101
    QC_StatusSubmittingSubmitting
    ID33213322




    Data Submission Information
    DCC submission information was not avaliable




    File Information
    file_namefile_pathfile_formatoutput_typeassay_typelabSample IDplatformread_lengthrun_typepaired_endassemblygenome_annotationderived_fromcontrolled_byProjectNoteFILE_ID
    NT-BGHcLV45-1_S1_L001_R1_001.filtered.trimmed.paired.fastq.gzENCODE_DATA/set76/fastq_trimmedfastqreadsknockdown followed by RNA-seqGraveley Lab3321NovaSeq6000100paired-ended1ENCORE231832
    NT-BGHcLV45-1_S1_L001_R2_001.filtered.trimmed.paired.fastq.gzENCODE_DATA/set76/fastq_trimmedfastqreadsknockdown followed by RNA-seqGraveley Lab3321NovaSeq6000100paired-ended2ENCORE231874
    NT-BGHcLV45-1_Aligned.sortedByCoord.out.bamENCODE_DATA/set76/STAR_ENCORE2_trimmed_file_linksbamalignmentsknockdown followed by RNA-seqGraveley Lab3321GRCh38V40NT-BGHcLV45-1_S1_L001_R1_001.filtered.trimmed.paired.fastq.gz,NT-BGHcLV45-1_S1_L001_R2_001.filtered.trimmed.paired.fastq.gzENCORE231916
    NT-BGHcLV45-1_Signal.UniqueMultiple.strand-.bwENCODE_DATA/set76/STAR_ENCORE2_trimmed_file_linksbigWig-allminus strand signal of all readsknockdown followed by RNA-seqGraveley Lab3321GRCh38V40NT-BGHcLV45-1_Aligned.sortedByCoord.out.bamENCORE231958
    NT-BGHcLV45-1_Signal.UniqueMultiple.strand+.bwENCODE_DATA/set76/STAR_ENCORE2_trimmed_file_linksbigWig+allplus strand signal of all readsknockdown followed by RNA-seqGraveley Lab3321GRCh38V40NT-BGHcLV45-1_Aligned.sortedByCoord.out.bamENCORE232000
    NT-BGHcLV45-1_Signal.Unique.strand-.bwENCODE_DATA/set76/STAR_ENCORE2_trimmed_file_linksbigWig-uniqueminus strand signal of unique readsknockdown followed by RNA-seqGraveley Lab3321GRCh38V40NT-BGHcLV45-1_Aligned.sortedByCoord.out.bamENCORE232042
    NT-BGHcLV45-1_Signal.Unique.strand+.bwENCODE_DATA/set76/STAR_ENCORE2_trimmed_file_linksbigWig+uniqueplus strand signal of unique readsknockdown followed by RNA-seqGraveley Lab3321GRCh38V40NT-BGHcLV45-1_Aligned.sortedByCoord.out.bamENCORE232084
    NT-BGHcLV45-1_quant.sfENCODE_DATA/set76/salmon_ENCORE2_file_linkstranscript_quantificationtranscript quantificationsknockdown followed by RNA-seqGraveley Lab3321GRCh38V40NT-BGHcLV45-1_S1_L001_R1_001.filtered.trimmed.paired.fastq.gz,NT-BGHcLV45-1_S1_L001_R2_001.filtered.trimmed.paired.fastq.gzENCORE232126
    NT-BGHcLV45-2_S2_L001_R1_001.filtered.trimmed.paired.fastq.gzENCODE_DATA/set76/fastq_trimmedfastqreadsknockdown followed by RNA-seqGraveley Lab3322NovaSeq6000100paired-ended1ENCORE231833
    NT-BGHcLV45-2_S2_L001_R2_001.filtered.trimmed.paired.fastq.gzENCODE_DATA/set76/fastq_trimmedfastqreadsknockdown followed by RNA-seqGraveley Lab3322NovaSeq6000100paired-ended2ENCORE231875
    NT-BGHcLV45-2_Aligned.sortedByCoord.out.bamENCODE_DATA/set76/STAR_ENCORE2_trimmed_file_linksbamalignmentsknockdown followed by RNA-seqGraveley Lab3322GRCh38V40NT-BGHcLV45-2_S2_L001_R1_001.filtered.trimmed.paired.fastq.gz,NT-BGHcLV45-2_S2_L001_R2_001.filtered.trimmed.paired.fastq.gzENCORE231917
    NT-BGHcLV45-2_Signal.UniqueMultiple.strand-.bwENCODE_DATA/set76/STAR_ENCORE2_trimmed_file_linksbigWig-allminus strand signal of all readsknockdown followed by RNA-seqGraveley Lab3322GRCh38V40NT-BGHcLV45-2_Aligned.sortedByCoord.out.bamENCORE231959
    NT-BGHcLV45-2_Signal.UniqueMultiple.strand+.bwENCODE_DATA/set76/STAR_ENCORE2_trimmed_file_linksbigWig+allplus strand signal of all readsknockdown followed by RNA-seqGraveley Lab3322GRCh38V40NT-BGHcLV45-2_Aligned.sortedByCoord.out.bamENCORE232001
    NT-BGHcLV45-2_Signal.Unique.strand-.bwENCODE_DATA/set76/STAR_ENCORE2_trimmed_file_linksbigWig-uniqueminus strand signal of unique readsknockdown followed by RNA-seqGraveley Lab3322GRCh38V40NT-BGHcLV45-2_Aligned.sortedByCoord.out.bamENCORE232043
    NT-BGHcLV45-2_Signal.Unique.strand+.bwENCODE_DATA/set76/STAR_ENCORE2_trimmed_file_linksbigWig+uniqueplus strand signal of unique readsknockdown followed by RNA-seqGraveley Lab3322GRCh38V40NT-BGHcLV45-2_Aligned.sortedByCoord.out.bamENCORE232085
    NT-BGHcLV45-2_quant.sfENCODE_DATA/set76/salmon_ENCORE2_file_linkstranscript_quantificationtranscript quantificationsknockdown followed by RNA-seqGraveley Lab3322GRCh38V40NT-BGHcLV45-2_S2_L001_R1_001.filtered.trimmed.paired.fastq.gz,NT-BGHcLV45-2_S2_L001_R2_001.filtered.trimmed.paired.fastq.gzENCORE232127