DHX29

General Information

Gene Name       : DHX29
Gene ID             : ENSG00000067248.9
Essential Gene : No

Functions

Localization(RBPImage: DHX29)

Domains (AlphaFold: DHX29)


Antibody Validation

Expression Level in Cell lines

Cell Line Gene Name Ensembl ID Rep1 TPM Rep2 TPM Average TPM Experiment ID
Gm12878DHX29ENSG00000067248.917.2515.7716.51ENCSR000AEF
HepG2DHX29ENSG00000067248.97.7914.3811.09ENCSR985KAT
K562DHX29ENSG00000067248.920.5420.8220.68ENCSR000AEO

Assays

0 Released experiments are available on ENCORE database.







  eCLIP  

No eCLIP experiment available



  Antibody Validation  
  GeneName    Antibody_DCC_ID    Product_ID    Lot_ID    Phosphate    HepG2 Primary Validation    K562 Primary Validation    HepG2 Secondary Validation    K562 Secondary Validation  
DHX295926S1Submitted, ENCORE2BGKcLV42-23, Submitted, ENCORE2


  KD RNA-Seq  
  GeneName    Cell_Line    Method    Exp_ID    Control_ID    Sample_reps    Control_reps    KD%    Status    Project    Note  
DHX29K562CRISPRDHX29-BGKcLV42-23,DHX29-BGKcLV42-24NT-BGKcLV42-1,NT-BGKcLV42-255.7,53.4SequencedENCORE2
DHX29HepG2CRISPRBGHcLV45-25,BGHcLV45-26BGHcLV45-1,BGHcLV45-20.0,0.0NotSatisfiedENCORE2


  RNA Bind-n-Seqn  

No RBNS experiment available



  Localization Image in HepG2  
    DHX29: Click to RBP Image Database
  • Nuclei
  • Nucleoplasm
  • Mutually Exclusive from Nucleolus
  • Cytoplasm
  • Compartment/Organelle
  • Mitochondria












    • RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
    DHX29Product_ID: 5926S
    Lot_ID: 1
    Source: Cell Signaling Technology
    Target Name: DHX29-human    		 CST_5926S_1_DHX29.png<br>Caption: IP-WB analysis of 5926S whole cell lysate using the DHX29 specific antibody, 5926S. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the DHX29-specific antibody, 5926S. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
    		 DHX29-K562-CRISPR-5926S.png<br>Caption: Western blot following CRISPR against DHX29 in K562 whole cell lysate using DHX29 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against DHX29. DHX29 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.








    RNA Targets

    • eCLIP assay for DHX29 is not avaliable on ENCORE database.



    Proteins bound to RNA
    • DHX29 RNA is bound by 9 proteins.  







    eCLIP assay for DHX29 is not avaliable on ENCORE database.





    9 proteins are bound to DHX29 RNA:

    Chr Start End Strand Matched Area log2(fold change) -log(p-value) Cell Line Binding Protein Name
    chr55457214354572203-CDS,intron-Distal,intron-Proximal4.150356.14226K562ZNF622
    chr55457733054577400-CDS,intron-Distal,intron-Proximal5.993208.42776K562SF3B4
    chr55457934854579408-CDS,intron-Distal4.728304.34038HepG2BCLAF1
    chr55458613054586173-CDS,intron-Distal,intron-Proximal4.730924.78529HepG2BCLAF1
    chr55457955854579635-CDS,intron-Distal4.617855.51215K562RBM15
    chr55458988454589931-CDS,intron-Distal4.406384.34038HepG2BCLAF1
    chr55458516654585221-CDS,intron-Distal3.456175.95375K562ZNF622
    chr55458984454589868-CDS,intron-Distal,intron-Proximal3.463303.17151K562UCHL5
    chr55458126454581500-intron-Distal,intron-Proximal4.083287.46973K562KHSRP
    chr55457734354577400-intron-Distal,intron-Proximal4.809684.51735HepG2QKI
    chr55460145354601596-intron-Distal4.276543.83958HepG2QKI
    chr55457742854577502-intron-Distal,intron-Proximal8.2354344.15781K562QKI
    chr55458477754584892-intron-Distal3.200265.62058K562CSTF2T
    chr55457740054577469-intron-Distal,intron-Proximal5.767755.89032HepG2QKI
    chr55457736054577428-intron-Distal,intron-Proximal6.8270632.43515K562QKI
    chr55457452254574582-intron-Distal4.374823.91475K562PTBP1
    chr55459176654591953-intron-Distal,intron-Proximal3.304845.57010K562KHSRP
    ChrStartEndStrandMatched Arealog2(fold change)-log(p-value)Cell LineBinding Protein Name




    eCLIP assay for DHX29 is not avaliable on ENCORE database.



    Diffrential Expression

    • The Knockdown/knockout assay for DHX29 is not available on ENCORE database.

    • 96 RBPs can affect the levels of DHX29.  








    The Knockdown/knockout RNA-Seq assay for DHX29 is not available on ENCORE database.





    96 RBPs can affect the gene expression of DHX29 :

    Gene NameGene IDKDMeanCNMeanlog2(fold change) *-log(pval) **-log(padj) **Cell LineAffected by RBPBound by RBP
    DHX29ENSG00000067248.51102.39640488.243701.1749014.5462013.42520HepG2AKAP1
    DHX29ENSG00000067248.5699.64300890.39470-0.347803.321502.58040HepG2AUH
    DHX29ENSG00000067248.5868.359601133.49940-0.384402.628702.04350HepG2BOP1
    DHX29ENSG00000067248.5433.12210880.08590-1.022905.155503.80490HepG2CCAR1
    DHX29ENSG00000067248.5619.95460383.824800.691605.655504.57400HepG2CIRBP
    DHX29ENSG00000067248.5747.58300496.349800.590808.342707.30950HepG2CKAP4
    DHX29ENSG00000067248.5585.149301040.64470-0.830608.621007.46580HepG2CPSF6
    DHX29ENSG00000067248.5657.56910459.142400.518106.360205.17390HepG2DDX19B
    DHX29ENSG00000067248.5554.15420322.251300.782006.576805.58140HepG2DDX21
    DHX29ENSG00000067248.5883.22450467.753100.9169010.013908.88970HepG2DDX3X
    DHX29ENSG00000067248.5196.66650401.68590-1.030304.641503.85950HepG2DDX6
    DHX29ENSG00000067248.5694.61230474.960700.548306.048705.12120HepG2DKC1
    DHX29ENSG00000067248.5278.38860401.10180-0.526903.204502.45260HepG2DNAJC21
    DHX29ENSG00000067248.5684.47260357.234600.938009.162607.60400HepG2EEF2
    DHX29ENSG00000067248.5552.87040297.766100.892708.230506.31620HepG2EFTUD2
    DHX29ENSG00000067248.5476.55620289.969300.716604.866903.69410HepG2EIF3D
    DHX29ENSG00000067248.5479.35260304.057400.656604.781603.40680HepG2EIF3G
    DHX29ENSG00000067248.5569.25830426.936600.415002.170001.60100HepG2EIF4A3
    DHX29ENSG00000067248.5874.34460622.407400.490204.909803.84670HepG2EIF4G1
    DHX29ENSG00000067248.5379.97410596.23760-0.650005.519404.39040HepG2EIF4G2
    DHX29ENSG00000067248.5846.785301022.23130-0.271702.102001.56330HepG2ETF1
    DHX29ENSG00000067248.5955.53650663.088300.527002.866102.08260HepG2FIP1L1
    DHX29ENSG00000067248.5636.53370430.581400.563903.986203.16550HepG2FXR1
    DHX29ENSG00000067248.5428.56280258.172600.731105.038803.82850HepG2GEMIN5
    DHX29ENSG00000067248.5631.187601179.73530-0.902309.221208.09710HepG2GPKOW
    DHX29ENSG00000067248.5659.52020404.943500.703606.398505.47540HepG2HNRNPA1
    DHX29ENSG00000067248.5601.88310450.398600.418202.552201.88430HepG2HNRNPC
    DHX29ENSG00000067248.5640.25940832.52190-0.378803.593402.78500HepG2HNRNPF
    DHX29ENSG00000067248.5402.05870574.76750-0.515605.614704.68380HepG2HNRNPL
    DHX29ENSG00000067248.5728.227201125.83550-0.628505.539504.68050HepG2HNRNPLL
    DHX29ENSG00000067248.5628.37500457.749900.457002.567701.64970HepG2HNRNPM
    DHX29ENSG00000067248.5563.76620447.422700.333402.482901.90760HepG2HNRNPU
    DHX29ENSG00000067248.5614.23800819.80170-0.416503.410002.61280HepG2IGF2BP1
    DHX29ENSG00000067248.5631.39030406.139000.636504.919604.04220HepG2IGF2BP2
    DHX29ENSG00000067248.5778.27250381.008401.030404.119102.92290HepG2IGF2BP3
    DHX29ENSG00000067248.5347.65670453.81840-0.384502.909201.86970HepG2KIF1C
    DHX29ENSG00000067248.5588.09920366.921400.680505.400103.82440HepG2LARP7
    DHX29ENSG00000067248.5531.66720385.140500.465002.580101.41220HepG2LIN28B
    DHX29ENSG00000067248.5739.51770430.731000.779707.157706.11880HepG2MAGOH
    DHX29ENSG00000067248.5461.76210310.594300.572003.459001.66870HepG2MARK2
    DHX29ENSG00000067248.5938.86340433.170901.1159010.911709.72000HepG2MATR3
    DHX29ENSG00000067248.5467.66670303.460900.623902.468501.47280HepG2METAP2
    DHX29ENSG00000067248.5773.65630537.053200.526503.234102.36200HepG2MSI2
    DHX29ENSG00000067248.5800.62940443.398300.852407.319106.31210HepG2NCBP2
    DHX29ENSG00000067248.5496.70960625.64330-0.332902.434501.72140HepG2NOL12
    DHX29ENSG00000067248.5753.00440998.20790-0.406702.782302.03140HepG2NSUN2
    DHX29ENSG00000067248.5579.02420392.185600.562005.666904.84680HepG2PCBP1
    DHX29ENSG00000067248.5797.96040424.537700.910307.197305.59930HepG2PCBP2
    DHX29ENSG00000067248.5687.74970446.296700.623803.497802.13940HepG2PRPF6
    DHX29ENSG00000067248.5630.41290460.541800.452903.220602.35540HepG2PRPF8
    DHX29ENSG00000067248.5708.26350399.053500.8276011.8125010.83800HepG2PTBP1
    DHX29ENSG00000067248.5442.37120597.21130-0.433003.087802.46400HepG2PUF60
    DHX29ENSG00000067248.5897.16450633.942800.500907.071406.21220HepG2PUM1
    DHX29ENSG00000067248.5748.36690505.655900.565507.503906.57250HepG2PUS1
    DHX29ENSG00000067248.5635.21930409.695700.632602.934502.20990HepG2RAVER1
    DHX29ENSG00000067248.5807.08150444.116600.8617013.1816012.16090HepG2NELFE
    DHX29ENSG00000067248.5672.07660413.619200.700208.571507.57590HepG2RECQL
    DHX29ENSG00000067248.5593.46920896.74960-0.595505.688504.75940HepG2RPLP0
    DHX29ENSG00000067248.5598.97440402.433300.573602.951101.97760HepG2RPS10
    DHX29ENSG00000067248.5474.80440282.179000.750606.381305.48940HepG2RPS19
    DHX29ENSG00000067248.5638.90040408.041400.646804.672103.64760HepG2RPS2
    DHX29ENSG00000067248.5412.23390291.153400.501602.263201.53850HepG2RPS3A
    DHX29ENSG00000067248.5566.47960416.550600.443402.322001.34260HepG2RPS5
    DHX29ENSG00000067248.5662.48190455.594500.540003.926903.01010HepG2RRP9
    DHX29ENSG00000067248.5291.24880398.46520-0.452202.327901.72880HepG2SAFB2
    DHX29ENSG00000067248.5466.67570283.169300.720705.305904.01390HepG2SERBP1
    DHX29ENSG00000067248.51099.47730662.509000.730705.531004.55870HepG2SF3A3
    DHX29ENSG00000067248.5564.08940415.484200.441003.438802.78830HepG2SF3B4
    DHX29ENSG00000067248.51183.17730433.668201.4479043.7055042.32150HepG2SRSF1
    DHX29ENSG00000067248.5795.710901198.96440-0.591503.799402.60840HepG2SRSF3
    DHX29ENSG00000067248.5632.65710817.90670-0.370503.452302.68670HepG2SRSF7
    DHX29ENSG00000067248.5243.22830352.80590-0.536603.950303.31450HepG2SRSF9
    DHX29ENSG00000067248.51177.56430837.927500.490807.825707.04880HepG2SSB
    DHX29ENSG00000067248.5954.69550670.597100.509506.979306.06980HepG2SUCLG1
    DHX29ENSG00000067248.5762.500901097.11980-0.524904.375903.49160HepG2TARDBP
    DHX29ENSG00000067248.5275.71870438.63480-0.669804.297103.62460HepG2TRIM56
    DHX29ENSG00000067248.51152.51930567.452701.0221011.5829010.26960HepG2U2AF1
    DHX29ENSG00000067248.5735.49610567.250000.374603.445902.79080HepG2UTP3
    DHX29ENSG00000067248.5593.24210402.862700.558202.857001.57430HepG2XRCC5
    DHX29ENSG00000067248.5925.61600450.550201.038609.785507.92610HepG2XRCC6
    DHX29ENSG00000067248.5531.33410646.18570-0.282302.282301.77110HepG2ZNF622
    DHX29ENSG00000067248.5943.710301179.49470-0.321803.077101.67440K562BCCIP
    DHX29ENSG00000067248.51487.347801761.72620-0.244202.000901.32570K562CPSF6
    DHX29ENSG00000067248.53021.747902132.915000.5025011.3481010.12620K562DDX47
    DHX29ENSG00000067248.51403.06160966.847900.537105.552304.23360K562EFTUD2
    DHX29ENSG00000067248.51509.334001974.28370-0.387402.353901.57170K562FIP1L1
    DHX29ENSG00000067248.51551.593501015.585800.6113011.2360010.10240K562FXR1
    DHX29ENSG00000067248.51525.342801132.926000.429002.663301.97250K562G3BP2
    DHX29ENSG00000067248.51382.313301682.01090-0.283102.161601.45750K562GPKOW
    DHX29ENSG00000067248.51851.985002393.92130-0.370301.952901.31210K562GRSF1
    DHX29ENSG00000067248.51655.477701229.213300.429403.553402.13510K562HNRNPK
    DHX29ENSG00000067248.52041.707701485.550900.458706.960205.53950K562HNRNPU
    DHX29ENSG00000067248.51934.291402676.86830-0.468703.833302.90000K562HSPD1
    DHX29ENSG00000067248.52091.407101619.185400.369103.149802.26550K562KIF1C
    DHX29ENSG00000067248.51199.496501641.83210-0.452904.763703.59610K562METAP2
    DHX29ENSG00000067248.51819.675701271.391700.517203.610102.56960K562NAA15
    DHX29ENSG00000067248.51712.128101150.806100.573007.123005.52670K562PCBP2
    DHX29ENSG00000067248.51445.452702241.03920-0.632606.430005.09020K562PPIG
    DHX29ENSG00000067248.51633.32120940.849400.7957011.210009.69160K562PRPF8
    DHX29ENSG00000067248.5583.90870924.80860-0.663406.798905.27220K562PSIP1
    DHX29ENSG00000067248.52406.714801513.219500.669308.675307.76260K562PUS1
    DHX29ENSG00000067248.51864.609001474.236700.338803.707503.03590K562RBM15
    DHX29ENSG00000067248.51247.15460958.501700.379702.626301.83290K562RPS19
    DHX29ENSG00000067248.52518.617101466.809600.779903.839602.30210K562SNRNP200
    DHX29ENSG00000067248.5590.27940799.69440-0.438102.708301.69850K562SRFBP1
    DHX29ENSG00000067248.51477.501301214.988100.282102.679401.53390K562SRSF7
    DHX29ENSG00000067248.51603.222401111.998600.527706.363205.11080K562SYNCRIP
    DHX29ENSG00000067248.5692.57250889.53640-0.361102.110801.30510K562TIAL1
    DHX29ENSG00000067248.5675.13670944.78590-0.484804.029402.84270K562TRIM56
    DHX29ENSG00000067248.51496.770601098.838400.445803.313802.09450K562U2AF1
    DHX29ENSG00000067248.52005.693601134.643400.8218017.7566015.67010K562U2AF2
    Gene NameGene IDKDMeanCNMeanlog2(fold change)-log(pval)-log(padj)Cell LineAffected by RBPBound by RBP

    *: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
    **: "-log(0)" was displayed as "99999.99999".






    The Knockdown/knockout RNA-Seq assay for DHX29 is not available on ENCORE database.





    2 RBPs can affect the splicing of DHX29 :

    Event TypeGene NameGene IDchrStrandExonStartExonEnd2ndExonStart2ndExonEndupstreamESupstreamEEdownstreamESdownstreamEEdPSIp-valueFDRis_includedCell LineAffected by RBPBound by RBP
    SEDHX29ENSG00000067248.5chr5-545556755455577254552072545523685456296554563059-0.089000.004210.04038excludedK562AQR
    SEDHX29ENSG00000067248.5chr5-545662395456631554565240545654655456640554566534-0.102000.000130.00213excludedK562AQR
    A3SSDHX29ENSG00000067248.5chr5-545691205456925454569120545692055457044154570559-0.141000.002880.08705shorterK562U2AF2
    A3SSDHX29ENSG00000067248.5chr5-5459311254593260545931125459322654594418545944920.168000.000010.00010longerK562AQR
    A3SSDHX29ENSG00000067248.5chr5-5457719954577431545771995457734354579030545796390.074000.000010.00012longerK562AQR
    A3SSDHX29ENSG00000067248.5chr5-5456640554566630545664055456653454567914545680390.157000.000000.00000longerK562AQR
    A3SSDHX29ENSG00000067248.5chr5-5458984454590083545898445458997354591206545913730.168000.000000.00000longerK562AQR
    A3SSDHX29ENSG00000067248.5chr5-5456791454568144545679145456800354569120545692050.212000.000000.00000longerK562AQR
    A3SSDHX29ENSG00000067248.5chr5-5458604554586293545860455458617254589844545899730.056000.000170.00144longerK562AQR
    A3SSDHX29ENSG00000067248.5chr5-5455719554557424545571955455732754558457545587600.131000.000000.00000longerK562AQR
    A3SSDHX29ENSG00000067248.5chr5-5458152354581760545815235458168954585097545852560.138000.000000.00002longerK562AQR
    A3SSDHX29ENSG00000067248.5chr5-5459127154591373545912715459135254592047545921770.276000.000000.00000longerK562AQR
    A3SSDHX29ENSG00000067248.5chr5-5456912054569254545691205456920554570441545705590.192000.003630.02181longerK562AQR
    A3SSDHX29ENSG00000067248.5chr5-545571955455742454557195545572725455845754558760-0.091000.020610.09780shorterK562AQR
    MXEDHX29ENSG00000067248.5chr5-54591206545913525459204754592177545898445458997354593112545932260.147000.007260.048891includedK562AQR
    MXEDHX29ENSG00000067248.5chr5-54567914545680035456912054569294545662715456653454570441545705590.056000.010050.063631includedK562AQR
    MXEDHX29ENSG00000067248.5chr5-54567914545680035456912054569254545662715456653454570441545705590.185000.000330.003461includedK562AQR
    MXEDHX29ENSG00000067248.5chr5-54567914545680365456912054569294545664055456653454570441545705590.060000.010230.064551includedK562AQR
    MXEDHX29ENSG00000067248.5chr5-54567914545680365456912054569254545662715456653454570441545705590.192000.000210.002271includedK562AQR
    MXEDHX29ENSG00000067248.5chr5-54581120545812445458154654581714545790305457963954585097545852560.096000.000010.000191includedK562AQR
    MXEDHX29ENSG00000067248.5chr5-54591206545913685459204754592177545898445458997354593112545932260.151000.010050.063591includedK562AQR

    *: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
    **: "-log(0)" was displayed as "99999.99999".





    The Knockdown/knockout RNA-Seq assay for DHX29 is not avaliable on ENCORE database.