SRRT      CRISPR in HepG2      Batch: BGHcLV45

Experiment Information (Status: Sequencing)
BGHcLV45-63BGHcLV45-64
idx12171217
TRCN#_or_BGC#BGC#0001062BGC#0001062
shRNA_or_gRNA_sequenceGAGAGATGAAAGACGTCGAGGAGAGATGAAAGACGTCGAG
PAMGGGGGG
NameSRRT_93SRRT_93
Sample_IDBGHcLV45-63BGHcLV45-64
transduction_Date10/1/2410/1/24
daysD6D6
RBP_nameSRRTSRRT
qPCR_result0.00.0
Ave_qPCR0.0
RT-qPCR_primer-Fgaggcaacagatgcaggatt
RT-qPCR_primer-Rtccatgagggacaggaagac
protein_conc31252276
WB_result93.991.2
Ave_WB
WB_DONE_date10/25/24
MW101kd
IP
antibody_Cat#A304-551Alot # 1
Antibody DCC ID
submitted_to_DCC_date
Rep1_TPM00
Rep2_TPM00
ActionReadyReady
Library_start_date
repeat_library
Note
ID77057706




Western Blot
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
SRRTProduct_ID: A304-551A
Lot_ID: 1
Source: Bethyl Labs
Target Name: SRRT-human		 SRRT-HEPG2-CRISPR-A304-551A.png<br>Caption: Western blot following CRISPR against SRRT in HepG2 whole cell lysate using SRRT specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against SRRT. SRRT protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
 SRRT-K562-CRISPR-A304-551A.png<br>Caption: Western blot following CRISPR against SRRT in K562 whole cell lysate using SRRT specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against SRRT. SRRT protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.




Experiment Status
BGHcLV45-63BGHcLV45-64
Sample_IDBGHcLV45-63BGHcLV45-64
Sample Name
Sample NameSRRT-BGHcLV45-63SRRT-BGHcLV45-64
RBPSRRTSRRT
Cell_LineHepG2HepG2
Exp UID
StatusSequencingSequencing
Status_date2024-12-122024-12-12
ProjectENCORE2ENCORE2
Note
ID1371413715




Library-Prep Information
BGHcLV45-63BGHcLV45-64
Sample #2930
Sample NameSRRT-BGHcLV45-63SRRT-BGHcLV45-64
Sample_Name_Alias
Index Well PositionE10F10
Index_tableIDT_UniqueDualIndex_96_V2IDT_UniqueDualIndex_96_V2
LibPrep_date2024-12-022024-12-02
Lib_IDLib-241202Lib-241202
Tecan_Location
Tecan
Tecan_date
Size_bp284296
Peak_Molarity66.9077.60
libSampleQC_DNA_Well
RIN
libSample_RNA_Well
SampleQC_methodTapeStation_2022TapeStation_2022
SampleQC_date2024-12-052024-12-05
Sample_IDBGHcLV45-63BGHcLV45-64
RBPSRRTSRRT
Batch_IDBGHcLV45BGHcLV45
WB_result93.90091.200
Library DescriptionTruSeq mRNATruSeq mRNA
Repeat_Library_Suffix
Lib_StatusSendToSequenceSendToSequence
ProjectENCORE2ENCORE2
ID46384639




Sequencing Information
Sequencing info was not avaliable




Data Submission Information
DCC submission information was not avaliable




File Information
Files of the experiment was not avaliable in the database