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IARS RNA-Seq in HepG2



IARS      CRISPR in HepG2      Control:

General Information
RBPIARS
Cell_LineHepG2
MethodCRISPR
Exp_NameIARS-BGHcLV41-HepG2
ENCODE_series_ID
Batch_IDBGHcLV41
Pool IDPool-240314
Local_Set_Nameset70
ENCODE_KD_Exp_ID
ENCODE_CN_Exp_ID
Rep1IARS-BGHcLV41-83
Rep2IARS-BGHcLV41-84
CN1NT-BGHcLV41-1
CN2NT-BGHcLV41-2
Rep1_qPCR65.1
Rep2_qPCR63.1
Rep1_WB77.9
Rep2_WB71.7
Antibody Cat#A304-748A
Antibody Lot#lot # 1
Antibody DCC ID
StatusSubmitted
ProjectENCORE2
ID1461




Experiment Information (Status: Submitting)
BGHcLV41-83BGHcLV41-83BGHcLV41-84BGHcLV41-84
idx1356135613571357
TRCN#_or_BGC#BGC#0001028BGC#0001028BGC#0001028BGC#0001028
shRNA_or_gRNA_sequenceTGGATACTCAAACATACCACTGGATACTCAAACATACCACTGGATACTCAAACATACCACTGGATACTCAAACATACCAC
PAMAGGAGGAGGAGG
NameIARS_84IARS_84IARS_84IARS_84
Sample_IDBGHcLV41-83BGHcLV41-83BGHcLV41-84BGHcLV41-84
transduction_Date1/12/241/12/241/12/241/12/24
daysD6D6D6D6
RBP_nameIARSIARSIARSIARS
qPCR_result65.165.163.163.1
Ave_qPCR64.164.1
RT-qPCR_primer-Fgccgatggattgactttgacgccgatggattgactttgac
RT-qPCR_primer-Raagggcatgactttcacaccaagggcatgactttcacacc
protein_conc2966296635973597
WB_result77.981.571.781.7
Ave_WB74.881.6
WB_DONE_date2/1/246/28/24
MW145kd145kd
IP
antibody_Cat#A304-748AA304-747Alot # 1lot # 1
Antibody DCC ID
submitted_to_DCC_date
Rep1_TPM0000
Rep2_TPM0000
ActionReadyReady
Library_start_date
repeat_library
Note
ID7551755375527554




Western Blot
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
IARSProduct_ID: A304-748A
Lot_ID: 1
Source: Bethyl Labs
Target Name: IARS-human		 IARS-HEPG2-CRISPR-A304-748A.png<br>Caption: Western blot following CRISPR against IARS in HepG2 whole cell lysate using IARS specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against IARS. IARS protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.




Experiment Status
BGHcLV41-83BGHcLV41-84
Sample_IDBGHcLV41-83BGHcLV41-84
Sample Name
Sample NameIARS-BGHcLV41-83IARS-BGHcLV41-84
RBPIARSIARS
Cell_LineHepG2HepG2
Exp UID
StatusSubmittingSubmitting
Status_date2024-09-202024-09-20
ProjectENCORE2ENCORE2
Note
ID1298212983




Library-Prep Information
BGHcLV41-83BGHcLV41-84
Sample #3536
Sample NameIARS-BGHcLV41-83IARS-BGHcLV41-84
Sample_Name_Alias
Index Well PositionC05D05
Index_tableIDT_UniqueDualIndex_96IDT_UniqueDualIndex_96
LibPrep_date2024-03-012024-03-01
Lib_IDLib-240301Lib-240301
Tecan_Location
Tecan
Tecan_date
Size_bp294299
Peak_Molarity78.3099.00
libSampleQC_DNA_WellDNA_Library_set70/set1/C5.pngDNA_Library_set70/set1/D5.png
RIN9.99.9
libSample_RNA_WellRNA_Library_set70/set1/G10.pngRNA_Library_set70/set1/H10.png
SampleQC_methodTapeStation_2022TapeStation_2022
SampleQC_date2024-03-142024-03-14
Sample_IDBGHcLV41-83BGHcLV41-84
RBPIARSIARS
Batch_IDBGHcLV41BGHcLV41
WB_result77.90071.700
Library DescriptionTruSeq mRNATruSeq mRNA
Repeat_Library_Suffix
Lib_StatusSendToSequenceSendToSequence
ProjectENCORE2ENCORE2
ID42604261




Sequencing Information
BGHcLV41-83BGHcLV41-84
Sample_IDBGHcLV41-83BGHcLV41-84
Sample NameIARS-BGHcLV41-83IARS-BGHcLV41-84
Pool IDPool-240314Pool-240314
LocalServer_folderset70set70
total_reads40,770,07329,750,658
total_aligned_reads37,795,54028,322,154
unique_aligned_reads35,833,78826,801,576
percent_uniqueAligned0.878920.90087
correlation_replicates0.9884430.988443
spikein_reads3,3341,296
percent_spikeins0.000080.00004
original_ReadLength151151
QC_StatusSubmittingSubmitting
ID29792980




Data Submission Information
DCC submission information was not avaliable




File Information
Files of the experiment was not avaliable in the database