GEMIN2

General Information

Gene Name       : GEMIN2
Gene ID             : ENSG00000092208.17
Essential Gene : Yes

Functions

Localization

Domains (AlphaFold: GEMIN2)


Antibody Validation

Expression Level in Cell lines

Cell Line Gene Name Ensembl ID Rep1 TPM Rep2 TPM Average TPM Experiment ID
Gm12878GEMIN2ENSG00000092208.1723.5625.8924.73ENCSR000AEF
HepG2GEMIN2ENSG00000092208.1751.2978.6264.95ENCSR985KAT
K562GEMIN2ENSG00000092208.1727.2930.6228.95ENCSR000AEO

Assays

2 Released experiments are available on ENCORE database.

Assay TypeExp_NameENCODE IDTarget labelBiosampleStatusLinked AntibodyControlsMethodNote
knockdown followed by RNA-seq GEMIN2-BGKcLV17-K562ENCSR039SOIGEMIN2K562ReleasedENCSR516TLX   CRISPRKD% = 89.9,90.2
knockdown followed by RNA-seq GEMIN2-BGHcLV18-HepG2ENCSR333OGAGEMIN2HepG2ReleasedENCSR670KAS   CRISPRKD% = 87.9,89.5






  eCLIP  

No eCLIP experiment available



  Antibody Validation  
  GeneName    Antibody_DCC_ID    Product_ID    Lot_ID    Phosphate    HepG2 Primary Validation    K562 Primary Validation    HepG2 Secondary Validation    K562 Secondary Validation  
GEMIN2ENCAB284NOXRN102PW1NotSatisfied, ENCODE4BGHcLV18-25, Released, ENCODE4BGKcLV17-25, Released, ENCODE4


  KD RNA-Seq  
  GeneName    Cell_Line    Method    Exp_ID    Control_ID    Sample_reps    Control_reps    KD%    Status    Project    Note  
GEMIN2K562CRISPRENCSR039SOIENCSR516TLXGEMIN2-BGKcLV17-25,GEMIN2-BGKcLV17-26NT-BGKcLV17-1,NT-BGKcLV17-289.9,90.2ReleasedENCODE4
GEMIN2HepG2CRISPRENCSR333OGAENCSR670KASGEMIN2-BGHcLV18-25,GEMIN2-BGHcLV18-26NT-BGHcLV18-1,NT-BGHcLV18-287.9,89.5ReleasedENCODE4


  RNA Bind-n-Seqn  

No RBNS experiment available



  Localization Image in HepG2  

No Localization Information available













RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
GEMIN2Product_ID: RN102PW
Lot_ID: 1
Source: MBLI
Target Name: GEMIN2-human		 GEMIN2-HEPG2-CRISPR-RN102PW.png<br>Caption: Western blot following CRISPR against GEMIN2 in HepG2 whole cell lysate using GEMIN2 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against GEMIN2. GEMIN2 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
 MBL_RN102PW_001_GEMIN2.png<br>Caption: IP-WB analysis of K562 whole cell lysate using the GEMIN2 specific antibody, RN102PW. Lane 1 is 2.5% of five million whole cell lysate input. Lanes 2 and 3 are 50% of IP enrichment from five million whole cell lysate using normal IgG antibody and the GEMIN2-specific antibody, RN102PW. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
 GEMIN2-K562-CRISPR-RN102PW.png<br>Caption: Western blot following CRISPR against GEMIN2 in K562 whole cell lysate using GEMIN2 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against GEMIN2. GEMIN2 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.








RNA Targets

  • eCLIP assay for GEMIN2 is not avaliable on ENCORE database.



Proteins bound to RNA
  • GEMIN2 RNA is bound by 7 proteins.  







eCLIP assay for GEMIN2 is not avaliable on ENCORE database.





7 proteins are bound to GEMIN2 RNA:

Chr Start End Strand Matched Area log2(fold change) -log(p-value) Cell Line Binding Protein Name
chr143960575039605829+3'-UTR3.913103.43253K562YBX3
chr143959168139591731+5'-UTR,CDS3.499724.24993HepG2PPIG
chr143959164339591744+5'-UTR,CDS3.644399.83277HepG2BCLAF1
chr143958365339583717+CDS,intron-Distal,intron-Proximal4.334295.93840HepG2PRPF8
chr143958354539583659+CDS,intron-Proximal3.957048.02029HepG2GRWD1
chr143958352639583679+CDS,intron-Proximal3.080513.38111K562DDX3X
chr143959313839593236+intron-Distal3.380044.56790HepG2ILF3
ChrStartEndStrandMatched Arealog2(fold change)-log(p-value)Cell LineBinding Protein Name




eCLIP assay for GEMIN2 is not avaliable on ENCORE database.



Diffrential Expression

  • The Knockdown/knockout assay for GEMIN2 is not available on ENCORE database.

  • 81 RBPs can affect the levels of GEMIN2.  








The Knockdown/knockout RNA-Seq assay for GEMIN2 is not available on ENCORE database.





81 RBPs can affect the gene expression of GEMIN2 :

Gene NameGene IDKDMeanCNMeanlog2(fold change) *-log(pval) **-log(padj) **Cell LineAffected by RBPBound by RBP
GEMIN2ENSG00000092208.121143.33250670.905900.769007.061806.25450HepG2AKAP1
GEMIN2ENSG00000092208.12831.457201007.82420-0.277502.365401.72170HepG2AUH
GEMIN2ENSG00000092208.12249.62960491.55770-0.977605.306804.09930HepG2BCLAF1
GEMIN2ENSG00000092208.12226.31780390.63420-0.787505.840305.00950HepG2BOP1
GEMIN2ENSG00000092208.12267.71160399.65110-0.578103.410902.05300HepG2BUD13
GEMIN2ENSG00000092208.12363.95160541.52790-0.573306.267005.33900HepG2CKAP4
GEMIN2ENSG00000092208.12198.49670358.61230-0.853306.031405.05150HepG2CPSF6
GEMIN2ENSG00000092208.12306.29350439.30340-0.520302.992701.84660HepG2CPSF7
GEMIN2ENSG00000092208.12378.08550556.08190-0.556602.353501.63370HepG2DDX55
GEMIN2ENSG00000092208.12226.70590407.23430-0.845004.022302.85780HepG2DDX59
GEMIN2ENSG00000092208.12361.86910518.15460-0.517904.422203.60240HepG2DKC1
GEMIN2ENSG00000092208.12267.96830350.36950-0.386802.514101.88660HepG2DNAJC2
GEMIN2ENSG00000092208.12275.59950530.73850-0.945408.355407.36350HepG2EIF2S1
GEMIN2ENSG00000092208.12481.63710735.01810-0.609805.337104.22660HepG2EIF4G2
GEMIN2ENSG00000092208.12803.439201157.12170-0.526307.178106.28550HepG2ETF1
GEMIN2ENSG00000092208.12183.38180359.25030-0.970103.490602.34540HepG2FTO
GEMIN2ENSG00000092208.12318.60450461.62530-0.535002.576401.49830HepG2FUS
GEMIN2ENSG00000092208.12258.86700406.48420-0.651003.730502.99620HepG2GPKOW
GEMIN2ENSG00000092208.12584.43760714.77530-0.290402.182301.34220HepG2HNRNPA2B1
GEMIN2ENSG00000092208.12361.84270546.01560-0.593603.690502.87200HepG2HNRNPF
GEMIN2ENSG00000092208.12506.95160708.49480-0.482905.682404.74680HepG2HNRNPL
GEMIN2ENSG00000092208.12143.27530387.91810-1.4370013.8934012.62860HepG2HNRNPLL
GEMIN2ENSG00000092208.12322.08910537.67240-0.739307.058705.94960HepG2IGF2BP1
GEMIN2ENSG00000092208.12397.30590558.01730-0.490102.953302.25940HepG2IGF2BP2
GEMIN2ENSG00000092208.12254.23740402.84770-0.664106.571605.48920HepG2ILF2
GEMIN2ENSG00000092208.12380.74060561.24680-0.559802.616401.64370HepG2KHSRP
GEMIN2ENSG00000092208.12198.35640305.00160-0.620702.677201.45370HepG2KRR1
GEMIN2ENSG00000092208.12257.53270395.69330-0.619602.552601.62530HepG2MTPAP
GEMIN2ENSG00000092208.12438.74140609.29920-0.473802.783502.14890HepG2NCBP2
GEMIN2ENSG00000092208.12601.97300771.23010-0.357502.929902.14510HepG2NOL12
GEMIN2ENSG00000092208.12263.98930510.42400-0.951204.583703.15640HepG2NONO
GEMIN2ENSG00000092208.12227.53450343.98590-0.596303.080502.29230HepG2NSUN2
GEMIN2ENSG00000092208.12289.74420390.38930-0.430102.275901.50140HepG2PARN
GEMIN2ENSG00000092208.12424.06750550.01050-0.375202.723402.12010HepG2PCBP1
GEMIN2ENSG00000092208.12287.47830436.98820-0.604103.013301.93000HepG2PCBP2
GEMIN2ENSG00000092208.12215.40350351.75730-0.707505.755204.72630HepG2PPIL4
GEMIN2ENSG00000092208.12742.77640459.375700.693204.274502.77560HepG2PRPF6
GEMIN2ENSG00000092208.12289.45810429.19160-0.568302.216901.49130HepG2PRPF8
GEMIN2ENSG00000092208.12426.92420559.70030-0.390702.734702.18960HepG2PTBP1
GEMIN2ENSG00000092208.12435.85720736.19830-0.756208.418607.45480HepG2PUF60
GEMIN2ENSG00000092208.12605.66450780.93250-0.366702.876602.19350HepG2PUM2
GEMIN2ENSG00000092208.12404.65620551.63440-0.447004.079603.35710HepG2PUS1
GEMIN2ENSG00000092208.12376.85670550.08790-0.545604.054203.25140HepG2RBM25
GEMIN2ENSG00000092208.12292.83110588.14920-1.0061011.156909.94420HepG2RPLP0
GEMIN2ENSG00000092208.12159.69330271.22030-0.764204.489703.72710HepG2RPS19
GEMIN2ENSG00000092208.12238.54500386.48880-0.696203.789102.85260HepG2RPS3A
GEMIN2ENSG00000092208.12267.56530424.58080-0.666103.085102.26150HepG2RRP9
GEMIN2ENSG00000092208.12424.02540705.87950-0.7353011.217709.96270HepG2SLBP
GEMIN2ENSG00000092208.12226.91050427.21200-0.912805.190004.10930HepG2SNRNP70
GEMIN2ENSG00000092208.12920.786801092.34580-0.246502.262101.79410HepG2SRFBP1
GEMIN2ENSG00000092208.12739.79970608.183600.282502.218601.78260HepG2SRSF1
GEMIN2ENSG00000092208.12491.76650726.87700-0.563706.815005.89240HepG2SRSF5
GEMIN2ENSG00000092208.12336.00790536.43230-0.674907.430806.37860HepG2SRSF7
GEMIN2ENSG00000092208.12329.90440494.81750-0.584805.549704.81520HepG2SRSF9
GEMIN2ENSG00000092208.12736.59580948.41180-0.364603.308102.75870HepG2SSB
GEMIN2ENSG00000092208.12238.74150359.80480-0.591802.731901.49930HepG2STAU1
GEMIN2ENSG00000092208.12676.37240826.66930-0.289502.622202.01330HepG2SUCLG1
GEMIN2ENSG00000092208.12277.36660553.63460-0.997105.536304.63080HepG2SUPV3L1
GEMIN2ENSG00000092208.12175.08630378.18160-1.111009.566708.33530HepG2TARDBP
GEMIN2ENSG00000092208.12240.15370371.12060-0.627903.009301.97160HepG2TFIP11
GEMIN2ENSG00000092208.12139.70450383.27850-1.4560017.8952016.82720HepG2TIAL1
GEMIN2ENSG00000092208.12179.43070386.63390-1.1075013.0011011.90520HepG2TRIM56
GEMIN2ENSG00000092208.12485.95880719.21870-0.565605.206303.96780HepG2TUFM
GEMIN2ENSG00000092208.12563.64330761.27120-0.433602.514501.71860HepG2UBE2L3
GEMIN2ENSG00000092208.12301.09570402.61130-0.419202.920302.23290HepG2ZC3H8
GEMIN2ENSG00000092208.12460.70280365.675000.333201.923801.43450K562AGGF1
GEMIN2ENSG00000092208.12329.31510579.99030-0.816606.901106.04210K562AQR
GEMIN2ENSG00000092208.12109.22120165.76750-0.601902.066601.31850K562BOP1
GEMIN2ENSG00000092208.1255.12820120.18650-1.124404.699503.24660K562CCAR1
GEMIN2ENSG00000092208.12172.89200244.00100-0.497002.297901.35250K562DDX3X
GEMIN2ENSG00000092208.12441.29100348.585800.340102.171901.58280K562DDX47
GEMIN2ENSG00000092208.12192.25730268.03930-0.479402.538401.39190K562EIF2S1
GEMIN2ENSG00000092208.12270.58680354.24260-0.388602.121101.47730K562EIF2S2
GEMIN2ENSG00000092208.12171.90330254.93030-0.568502.772701.95020K562EIF4A3
GEMIN2ENSG00000092208.12470.98570648.28560-0.460903.724202.63640K562EIF4G2
GEMIN2ENSG00000092208.1297.58710179.34730-0.878003.928102.90650K562FMR1
GEMIN2ENSG00000092208.1278.88940210.97480-1.419209.521008.46670K562HNRNPC
GEMIN2ENSG00000092208.12317.45230187.847100.756902.901001.94490K562HNRNPM
GEMIN2ENSG00000092208.12476.44830309.588600.621904.333903.34440K562HSPD1
GEMIN2ENSG00000092208.12164.41810235.73750-0.519802.014101.30530K562PCBP3
GEMIN2ENSG00000092208.12119.43880180.81840-0.598302.475401.72040K562POLR2G
GEMIN2ENSG00000092208.12236.23250343.81560-0.541403.510202.77340K562PTBP1
GEMIN2ENSG00000092208.12572.47100737.14710-0.364702.943301.95510K562PUM2
GEMIN2ENSG00000092208.12226.33340532.21800-1.233602.661701.39360K562SFPQ
GEMIN2ENSG00000092208.1296.72460163.68220-0.758902.926401.86140K562SRFBP1
GEMIN2ENSG00000092208.12254.98140181.366000.491402.298601.54950K562SYNCRIP
GEMIN2ENSG00000092208.12210.68080341.10860-0.695204.488803.58240K562TARDBP
GEMIN2ENSG00000092208.12119.64990181.94710-0.604702.189901.36690K562TIAL1
GEMIN2ENSG00000092208.12606.85930797.53920-0.394202.852501.42590K562UBE2L3
GEMIN2ENSG00000092208.12215.92250287.56330-0.413402.079301.45000K562UPF1
Gene NameGene IDKDMeanCNMeanlog2(fold change)-log(pval)-log(padj)Cell LineAffected by RBPBound by RBP

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".






The Knockdown/knockout RNA-Seq assay for GEMIN2 is not available on ENCORE database.





7 RBPs can affect the splicing of GEMIN2 :

Event TypeGene NameGene IDchrStrandExonStartExonEnd2ndExonStart2ndExonEndupstreamESupstreamEEdownstreamESdownstreamEEdPSIp-valueFDRis_includedCell LineAffected by RBPBound by RBP
SEGEMIN2ENSG00000092208.12chr14+3960286439602923396011613960127239605643396061770.098000.000050.00543includedHepG2RBM22
SEGEMIN2ENSG00000092208.12chr14+396028643960292339601161396012723960564339606177-0.157000.000680.01486excludedHepG2U2AF1
SEGEMIN2ENSG00000092208.12chr14+395877433958780339587202395872923959163339591747-0.057000.001180.02355excludedHepG2U2AF1
SEGEMIN2ENSG00000092208.12chr14+396028643960292339601161396012723960564339606177-0.130000.004510.04897excludedHepG2U2AF2
SEGEMIN2ENSG00000092208.12chr14+395877433958780339587202395872923959163339591747-0.104000.000000.00000excludedHepG2U2AF2
SEGEMIN2ENSG00000092208.12chr14+3959419539594240395916333959174739597483395975520.086000.001520.07775includedHepG2AKAP1
SEGEMIN2ENSG00000092208.12chr14+395877293958780339587202395872923959163339591747-0.061000.002150.02333excludedK562AQR
SEGEMIN2ENSG00000092208.12chr14+395941953959424039591633395917473959748339597552-0.141000.000610.03260excludedHepG2PCBP1
SEGEMIN2ENSG00000092208.12chr14+396028643960292339601161396012723960564339606177-0.256000.000000.00001excludedK562U2AF2
MXEGEMIN2ENSG00000092208.12chr14+39594177395942403959748339597552395916333959174739601161396012720.100000.002150.017721includedK562AQR
MXEGEMIN2ENSG00000092208.12chr14+3958774339587803395916333959174739587202395872923959748339597552-0.134000.002390.019312includedK562AQR
MXEGEMIN2ENSG00000092208.12chr14+3959748339597552396011613960127239591633395917473960564339605700-0.369000.000410.030072includedHepG2AKAP1
MXEGEMIN2ENSG00000092208.12chr14+39591633395917473959419539594240395877433958780339597483395975520.129000.002270.059661includedHepG2HNRNPU

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".





The Knockdown/knockout RNA-Seq assay for GEMIN2 is not avaliable on ENCORE database.