CHTOP

General Information

Gene Name       : CHTOP
Gene ID             : ENSG00000160679.12
Essential Gene : No

Functions

Localization

Domains (AlphaFold: CHTOP)


Antibody Validation

Expression Level in Cell lines

Cell Line Gene Name Ensembl ID Rep1 TPM Rep2 TPM Average TPM Experiment ID
Gm12878CHTOPENSG00000160679.1229.9435.0732.51ENCSR000AEF
HepG2CHTOPENSG00000160679.1235.1436.0835.61ENCSR985KAT
K562CHTOPENSG00000160679.1249.3545.8547.60ENCSR000AEO

Assays

2 Released experiments are available on ENCORE database.

Assay TypeExp_NameENCODE IDTarget labelBiosampleStatusLinked AntibodyControlsMethodNote
knockdown followed by RNA-seq CHTOP-BGKcLV21-K562ENCSR913OZWCHTOPK562ReleasedENCSR854OFV   CRISPRKD% = 78.5,73.9
knockdown followed by RNA-seq CHTOP-BGHcLV22-HepG2ENCSR298AQXCHTOPHepG2ReleasedENCSR704VPL   CRISPRKD% = 79.4,82.2






  eCLIP  

No eCLIP experiment available



  Antibody Validation  
  GeneName    Antibody_DCC_ID    Product_ID    Lot_ID    Phosphate    HepG2 Primary Validation    K562 Primary Validation    HepG2 Secondary Validation    K562 Secondary Validation  
CHTOPENCAB775SVWA303-411A1BGHcLV22-7, Released, ENCODE4BGKcLV21-5, Released, ENCODE4


  KD RNA-Seq  
  GeneName    Cell_Line    Method    Exp_ID    Control_ID    Sample_reps    Control_reps    KD%    Status    Project    Note  
CHTOPK562CRISPRENCSR913OZWENCSR854OFVCHTOP-BGKcLV21-5,CHTOP-BGKcLV21-6NT-BGKcLV21-1,NT-BGKcLV21-278.5,73.9ReleasedENCODE4
CHTOPHepG2CRISPRENCSR298AQXENCSR704VPLCHTOP-BGHcLV22-7,CHTOP-BGHcLV22-8NT-BGHcLV22-1,NT-BGHcLV22-279.4,82.2ReleasedENCODE4


  RNA Bind-n-Seqn  

No RBNS experiment available



  Localization Image in HepG2  

No Localization Information available













RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
CHTOPProduct_ID: A303-411A
Lot_ID: 1
Source: Bethyl Labs
Target Name: CHTOP-human		 CHTOP-HEPG2-CRISPR-A303-411A.png<br>Caption: Western blot following CRISPR against CHTOP in HepG2 whole cell lysate using CHTOP specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against CHTOP. CHTOP protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
 CHTOP-K562-CRISPR-A303-411A.png<br>Caption: Western blot following CRISPR against CHTOP in K562 whole cell lysate using CHTOP specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against CHTOP. CHTOP protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.








RNA Targets

  • eCLIP assay for CHTOP is not avaliable on ENCORE database.



Proteins bound to RNA
  • CHTOP RNA is bound by 26 proteins.  







eCLIP assay for CHTOP is not avaliable on ENCORE database.





26 proteins are bound to CHTOP RNA:

Chr Start End Strand Matched Area log2(fold change) -log(p-value) Cell Line Binding Protein Name
chr1153617972153617988+3'-UTR3.242416.23045K562TIA1
chr1153615047153615119+3'-UTR,intron-Distal3.692764.58287K562PRPF8
chr1153617988153618021+3'-UTR3.156687.08705K562TIA1
chr1153617899153617951+3'-UTR3.055093.18141HepG2FUBP3
chr1153618647153618767+3'-UTR4.493213.97692K562IGF2BP1
chr1153618124153618147+3'-UTR4.548678.58031HepG2U2AF2
chr1153617742153617812+3'-UTR3.983247.44026K562YBX3
chr1153615828153615842+3'-UTR,CDS,intron-Distal,intron-Proximal3.897333.23171K562FUS
chr1153618252153618349+3'-UTR3.975334.24993K562HNRNPL
chr1153618112153618174+3'-UTR3.391114.46214K562U2AF2
chr1153614878153614949+3'-UTR,CDS,intron-Distal,intron-Proximal4.0659421.42455HepG2PRPF8
chr1153617968153618012+3'-UTR3.064205.46818K562U2AF1
chr1153614911153615000+3'-UTR,CDS,intron-Distal,intron-Proximal3.787884.79018HepG2EFTUD2
chr1153617746153617824+3'-UTR3.7644410.50538K562PUM2
chr1153615810153615828+3'-UTR,CDS,intron-Distal3.799143.23171K562FUS
chr1153615608153615680+3'-UTR,intron-Distal,intron-Proximal3.902568.94372HepG2QKI
chr1153614904153614950+3'-UTR,CDS,intron-Distal,intron-Proximal5.9845612.64691K562AQR
chr1153610769153610922+5'-UTR,CDS,intron-Proximal3.227849.69039HepG2GRWD1
chr1153606554153606601+5'-UTR4.574987.70265K562RBM15
chr1153617592153617670+CDS3.442599.43454K562ZC3H11A
chr1153617664153617721+CDS4.160704.15341HepG2ZC3H11A
chr1153614703153614743+CDS,intron-Distal,intron-Proximal3.910833.74557K562U2AF2
chr1153617523153617630+CDS,intron-Proximal4.0630618.88190HepG2U2AF1
chr1153617536153617619+CDS,intron-Proximal3.2380111.27038K562DDX6
chr1153617539153617626+CDS,intron-Proximal3.849065.63879HepG2RPS3
chr1153617566153617671+CDS3.640138.04296K562RPS3
chr1153609120153609168+CDS,intron-Proximal4.7686712.43500HepG2PRPF8
chr1153617521153617614+CDS,intron-Proximal3.3841716.46151K562U2AF1
chr1153617523153617618+CDS,intron-Proximal3.6381324.37838K562U2AF2
chr1153614801153614917+CDS,intron-Distal,intron-Proximal3.223457.84823HepG2PPIG
chr1153617549153617627+CDS3.148337.17530HepG2SRSF9
chr1153614704153614808+CDS,intron-Distal,intron-Proximal3.5552513.48311HepG2U2AF2
chr1153617521153617626+CDS,intron-Proximal3.8607651.21148HepG2U2AF2
chr1153617405153617481+intron-Distal,intron-Proximal6.2707924.67472K562U2AF2
chr1153614545153614593+intron-Distal3.552383.39857HepG2HNRNPM
chr1153610446153610545+intron-Distal3.674605.19856HepG2PTBP1
chr1153617402153617456+intron-Distal,intron-Proximal5.7582011.76568K562U2AF1
chr1153609397153609529+intron-Distal5.9815718.43729HepG2EIF3H
chr1153617386153617488+intron-Distal,intron-Proximal4.7394344.55765HepG2U2AF2
chr1153617397153617481+intron-Distal,intron-Proximal3.905776.64882HepG2U2AF1
chr1153609481153609523+intron-Distal4.226403.48187K562RBM15
chr1153611103153611159+intron-Distal3.151575.19836HepG2U2AF2
chr1153610931153611031+intron-Distal,intron-Proximal3.193853.04831K562RBM15
chr1153609370153609484+intron-Distal3.282453.55908K562SAFB
chr1153611115153611173+intron-Distal3.369823.37074K562TIA1
chr1153617312153617391+intron-Distal3.9885211.77769K562KHSRP
chr1153613759153613811+intron-Distal5.082066.95924HepG2HNRNPM
chr1153609129153609180+intron-Proximal3.507033.43213K562UCHL5
chr1153610924153610991+intron-Proximal4.310839.40826K562AQR
ChrStartEndStrandMatched Arealog2(fold change)-log(p-value)Cell LineBinding Protein Name




eCLIP assay for CHTOP is not avaliable on ENCORE database.



Diffrential Expression

  • The Knockdown/knockout assay for CHTOP is not available on ENCORE database.

  • 46 RBPs can affect the levels of CHTOP.  








The Knockdown/knockout RNA-Seq assay for CHTOP is not available on ENCORE database.





46 RBPs can affect the gene expression of CHTOP :

Gene NameGene IDKDMeanCNMeanlog2(fold change) *-log(pval) **-log(padj) **Cell LineAffected by RBPBound by RBP
CHTOPENSG00000160679.81753.043101166.277400.587904.978904.29150HepG2AKAP1
CHTOPENSG00000160679.81384.340301743.56020-0.332804.466303.63010HepG2AUH
CHTOPENSG00000160679.8797.734701040.38600-0.383102.711002.03010HepG2CPSF6
CHTOPENSG00000160679.81019.418701289.78560-0.339404.845603.90500HepG2DAZAP1
CHTOPENSG00000160679.81194.134301021.819500.224701.902301.32420HepG2DKC1
CHTOPENSG00000160679.8790.85230623.992600.341803.596402.87070HepG2DNAJC2
CHTOPENSG00000160679.81481.765201019.435600.539404.369503.57010HepG2EIF4A3
CHTOPENSG00000160679.81310.37820992.695400.400502.104001.50020HepG2EIF4B
CHTOPENSG00000160679.81303.757701043.658300.320902.658401.84020HepG2EWSR1
CHTOPENSG00000160679.8824.09730551.676800.578905.414404.32540HepG2FUBP3
CHTOPENSG00000160679.81365.465001028.776700.408402.998002.27640HepG2FXR1
CHTOPENSG00000160679.81567.57570954.231300.716007.636206.55730HepG2G3BP1
CHTOPENSG00000160679.81411.978601013.182500.478704.058702.97130HepG2G3BP2
CHTOPENSG00000160679.8824.89680635.601100.376002.636702.00340HepG2HNRNPA1
CHTOPENSG00000160679.8962.242801185.30700-0.300803.035802.06330HepG2HNRNPA2B1
CHTOPENSG00000160679.81375.30060966.502400.508804.817503.91660HepG2HNRNPK
CHTOPENSG00000160679.8867.02630717.386400.273202.675101.95440HepG2ILF2
CHTOPENSG00000160679.81703.575801253.958900.442002.813201.97690HepG2LARP4
CHTOPENSG00000160679.81342.255301029.136800.383102.599101.96280HepG2MAGOH
CHTOPENSG00000160679.81079.50910927.181600.219401.853401.33960HepG2PCBP1
CHTOPENSG00000160679.8657.11150494.069500.411303.169801.84760HepG2PKM
CHTOPENSG00000160679.81407.980701673.17910-0.249002.363901.87510HepG2RBM22
CHTOPENSG00000160679.81308.78340927.318900.497005.563204.63780HepG2RBM25
CHTOPENSG00000160679.81173.50450899.683500.383202.479301.54570HepG2RBM27
CHTOPENSG00000160679.8811.69150581.371500.481404.104503.37090HepG2RPS19
CHTOPENSG00000160679.81861.517301269.096800.552603.874203.06990HepG2SF3A3
CHTOPENSG00000160679.81169.89260982.225000.252202.352701.80180HepG2SF3B4
CHTOPENSG00000160679.8893.203701239.53630-0.472702.434801.70340HepG2SNRNP70
CHTOPENSG00000160679.81398.282901205.366100.214101.917001.38320HepG2SRSF5
CHTOPENSG00000160679.81745.204101057.121100.723209.501308.32330HepG2U2AF1
CHTOPENSG00000160679.83763.083203009.898100.322102.651302.07360K562AQR
CHTOPENSG00000160679.81345.540601793.42880-0.414504.758203.73390K562CPSF6
CHTOPENSG00000160679.82011.588101342.054500.583804.899103.15460K562EEF2
CHTOPENSG00000160679.81728.775801296.713900.414803.731002.65910K562EFTUD2
CHTOPENSG00000160679.81766.567801388.432300.347403.024702.16860K562EIF4A3
CHTOPENSG00000160679.81826.118501443.911600.338704.309203.57810K562FXR1
CHTOPENSG00000160679.81951.553902419.42590-0.310003.664102.63580K562HNRNPU
CHTOPENSG00000160679.81458.724201772.31020-0.280902.492801.73450K562POLR2G
CHTOPENSG00000160679.81987.952701261.850800.655608.315107.01380K562PRPF8
CHTOPENSG00000160679.83428.742203948.61910-0.203702.327001.44510K562PUM2
CHTOPENSG00000160679.81743.265201430.253600.285402.130501.39670K562RBM22
CHTOPENSG00000160679.81288.382801058.683800.283202.199101.31830K562RCC2
CHTOPENSG00000160679.82051.842501579.231300.377602.719401.64880K562SAFB
CHTOPENSG00000160679.81529.568401151.937600.409004.668303.54660K562SF3B4
CHTOPENSG00000160679.82063.005701462.400300.496306.564305.48620K562SRSF1
CHTOPENSG00000160679.81518.110401159.912800.388203.657102.42230K562SRSF4
CHTOPENSG00000160679.82156.115101777.699800.278302.518901.73730K562SYNCRIP
CHTOPENSG00000160679.81574.974901185.697200.409503.775702.63870K562TRIM56
CHTOPENSG00000160679.81372.084401109.014300.307002.741801.94090K562U2AF2
CHTOPENSG00000160679.81905.919702428.21250-0.349406.058905.04410K562UPF1
CHTOPENSG00000160679.81675.242102077.75670-0.310702.980302.07290K562WDR3
Gene NameGene IDKDMeanCNMeanlog2(fold change)-log(pval)-log(padj)Cell LineAffected by RBPBound by RBP

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".






The Knockdown/knockout RNA-Seq assay for CHTOP is not available on ENCORE database.





23 RBPs can affect the splicing of CHTOP :

Event TypeGene NameGene IDchrStrandExonStartExonEnd2ndExonStart2ndExonEndupstreamESupstreamEEdownstreamESdownstreamEEdPSIp-valueFDRis_includedCell LineAffected by RBPBound by RBP
SECHTOPENSG00000160679.8chr1+153615702153615840153614721153614905153617539153618782-0.169000.000030.00444excludedHepG2TBRG4
SECHTOPENSG00000160679.8chr1+1536147211536149051536107701536109241536175391536187780.129000.009680.08901includedHepG2U2AF2
SECHTOPENSG00000160679.8chr1+153615702153615840153614721153614905153617539153618782-0.080000.000000.00000excludedK562AQR
SECHTOPENSG00000160679.8chr1+153615702153615840153614721153614905153617539153618782-0.077000.003000.08863excludedK562FXR1
SECHTOPENSG00000160679.8chr1+153615644153615840153614721153614905153617539153618782-0.129000.000350.06755excludedK562APOBEC3C
SECHTOPENSG00000160679.8chr1+153615702153615840153614721153614905153617539153618782-0.096000.001170.03454excludedHepG2SF3A3
SECHTOPENSG00000160679.8chr1+153615644153615840153614721153614905153617539153618782-0.192000.000070.01899excludedK562EIF4G2
RICHTOPENSG00000160679.8chr1+153609047153610924153609047153609129153610770153610924-0.168000.000000.00000excludedHepG2AKAP1
RICHTOPENSG00000160679.8chr1+1536090471536109241536090471536091291536107701536109240.120000.000250.06603includedK562GEMIN5
RICHTOPENSG00000160679.8chr1+1536090471536109241536090471536091291536107701536109240.251000.000000.00000includedK562PUS1
RICHTOPENSG00000160679.8chr1+153609047153610924153609047153609129153610770153610924-0.304000.000420.00433excludedHepG2U2AF2
RICHTOPENSG00000160679.8chr1+1536090471536109241536090471536091291536107701536109240.115000.002960.03575includedHepG2PRPF8
RICHTOPENSG00000160679.8chr1+153609047153610924153609047153609129153610770153610924-0.071000.005060.08545excludedK562SRSF1
RICHTOPENSG00000160679.8chr1+153609047153610924153609047153609129153610770153610924-0.149000.000020.00140excludedHepG2MATR3
RICHTOPENSG00000160679.8chr1+1536090471536109241536090471536091291536107701536109240.113000.005010.05077includedHepG2QKI
RICHTOPENSG00000160679.8chr1+1536090471536109241536090471536091291536107701536109240.134000.000960.05037includedHepG2HNRNPA1
RICHTOPENSG00000160679.8chr1+1536090471536109241536090471536091291536107701536109240.112000.000310.00401includedK562AKAP8L
RICHTOPENSG00000160679.8chr1+1536090471536109241536090471536091291536107701536109240.116000.000260.02193includedK562WDR3
RICHTOPENSG00000160679.8chr1+1536090471536109241536090471536091291536107701536109240.109000.000630.00869includedK562RBM15
RICHTOPENSG00000160679.8chr1+153609047153610924153609047153609129153610770153610924-0.085000.000830.05540excludedK562EIF3G
RICHTOPENSG00000160679.8chr1+1536090471536109241536090471536091291536107701536109240.127000.002320.09632includedHepG2HNRNPU
RICHTOPENSG00000160679.8chr1+1536090471536109241536090471536091291536107701536109240.096000.002020.02178includedK562EFTUD2
RICHTOPENSG00000160679.8chr1+1536090471536109241536090471536091291536107701536109240.156000.000000.00001includedK562AQR
RICHTOPENSG00000160679.8chr1+153609047153610924153609047153609129153610770153610924-0.086000.002520.08851excludedHepG2LARP4
RICHTOPENSG00000160679.8chr1+153609047153610924153609047153609129153610770153610924-0.107000.000170.00451excludedK562FXR1
MXECHTOPENSG00000160679.8chr1+1536147211536149051536157021536158401536105591536109241536175391536179460.238000.000450.058571includedHepG2XRCC6

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".





The Knockdown/knockout RNA-Seq assay for CHTOP is not avaliable on ENCORE database.