| HepG2 | | 
Caption: Western blot following shRNA against PUF60 in K562 and HepG2 whole cell lysate using PUF60 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against PUF60. Lanes 5-8 follow the same pattern, but in HepG2. PUF60 protein appears as the green band, Tubulin serves as a control and appears in red. Releated Sample: BGKLV25-43 Status: Released Lab: Graveley Lab |
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| K562 | 
Caption: IP-Western Blot analysis of K562 whole cell lysate using PUF60 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-PUF60 antibody (lanes under 'PUF60'). Method: immunoprecipitation Status: Released Lab: Yeo Lab |
Caption: Western blot following shRNA against PUF60 in K562 and HepG2 whole cell lysate using PUF60 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against PUF60. Lanes 5-8 follow the same pattern, but in HepG2. PUF60 protein appears as the green band, Tubulin serves as a control and appears in red. Releated Sample: BGKLV25-43 Status: Released Lab: Graveley Lab |
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